Roche Diagnostics Operations, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Renate Kolb

Dieter Heindl

Waltraud Ankenbauer

Frank Laue

Eva Walter

Ulrike Dietrich-veenstra

Stephanie Wessner

Yvonne Maerz

The present disclosure is directed to the stabilization of nucleated cells in a whole blood sample ex-vivo, effected by an additive being a liquid composition for stabilizing an analyte in intact nucleated cells of a whole-blood sample ex-vivo, the composition being an aqueous solution, the solution comprising an anticoagulant, a phosphate salt, a cell-metabolizable sugar, adenine, and an antioxidant, wherein the antioxidant comprises a mitochondria-targeted antioxidant, preferably a mitochondria-targeted antioxidant selected from the group consisting of SkQ1, MitoQ, SS-31, and a mixture thereof. In a specific embodiment, the liquid composition further comprises a protease inhibitor. Further provided is advantageous use of the composition for stabilizing an analyte selected from DNA, RNA and protein in intact nucleated cells of the whole-blood sample ex-vivo, as well as kits including the composition for practicing said use. Additionally, specific methods are provided for stabilizing intact nucleated cells of a whole-blood sample ex-vivo.

Stabilization of whole blood at room temperature

The present invention is directed to a novel chemical compound comprising the structure [Xx-(CH2)m-phosphate-Yy]n, characterized in that 3&#x2266;m&#x2266;6, 30&#x2266;n&#x2266;60, each x and y is independently from each other 0 or 1, each X and Y is independently from each other any photometrically measurable entity; provided that the terminal X can also be an &#x2014;OH group or a phosphate group, and further provided that the terminal Y can also be an &#x2014;OH group. Such a compound can be used as a suitable hot start additive for PCR based amplification of nucleic acids.

Polyanion for improved nucleic acid amplification

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Iffeldorf, Germany

Renate Kolb