Thermo Fisher Scientific Baltics Uab

Fermentas Uab

Renata Rimseliene

Rasa Sukackaite

Remigijus Skirgaila

Arvydas Lubys

Mindaugas Ukanis

Skaiste Valinskyte

Arvydas Janulaitis

Agne Tubeleviciūté

Sigitas Burinskas

Karolis Matjosaitis

Martyna Simutyte

Aurelija Zvirbliene

Donata Ulcinaite

Birute Gagiliene

Indre Kucinskaite-Kodze

Dovile Dekaminaviciute

Remigijus Skirgalia

This disclosure relates to thermophilic family B DNA polymerases comprising a neutral amino acid residue at a certain position near the C-terminus of the catalytic domain, which corresponds to a position occupied by a basic amino acid residue in wild-type Pfu polymerase. Related uses, methods, and compositions are also provided. In some embodiments, the polymerases comprise a 3'-5&#x2032; exonuclease domain and/or a sequence non-specific dsDNA binding domain.

Thermophilic DNA polymerase mutants

This disclosure relates to antibodies that bind thermophilic family B DNA polymerases. Related uses, methods, and compositions are also provided.

Antibodies that bind thermophilic DNA polymerases

A restriction endonuclease with a recognition sequence 5'-TCGA-3&#x2032;. The restriction endonuclease is sensitive to the presence of a modified cytosine residue in the recognition sequence. Methods and kits using the restriction endonuclease with a recognition sequence 5&#x2032;-TCGA-3&#x2032; are also disclosed.

Restriction endonucleases and their uses

A process for producing a polynucleotide encoding a restriction endonuclease with an altered specificity, which process comprises:

Nuclease

Growing community of inventors

Vilnius, Lithuania

Renata Rimseliene