International Minerals & Chemical Corp.

Pitman-moore, Inc.

Biothera Inc.

Ren-der Yang

James Rolke

Andrew Magee

Eun K Lee

Galina Blum

Edwin J Hamilton, Jr

Alvin M Janski

Larry D Taber

Particulate &#x3b2;-glucan is solubilized at elevated pressure and temperature to form soluble &#x3b2;-glucan. The method is safe and economical and produces a product that is an improved pharmaceutical agent.

Glucan preparations

A method for recovering a recombinant protein from a protein solution containing high molecular weight contaminating proteins by directly adding Group IIA metal salts to the solution in amounts sufficient to selectively precipitate the high molecular weight protein contaminants is disclosed. The high molecular weight precipitates are removed and the solution is further processed to remove low molecular weight contaminating proteins and other non-protein contaminants. The recombinant protein is subsequently recovered and further processed to produce a protein composition suitable for its intended use.

Method for recovering recombinant proteins

This invention relates to a method for purifying and concentrating biologically active growth hormone to produce growth hormone in a form suitable for incorporation into a controlled release device (or system). A buffered solution of purified recombinant growth hormone is dialyzed against a buffered solution until the salt level is reduced to less than 5% and then lyophilized.

Cylindrical implants for the controlled release of growth hormones

Monomeric, biologically active growth hormone is isolated from microbially-produced insoluble inclusion bodies by solubilizing and denaturing the growth hormone by extraction of the inclusion bodies into a guanidine salt solution such as guanidine hydrochloride and subsequently renaturing at least a portion of the growth hormone in the solution by replacing the guanidine salt solution with a denaturant-free buffer solution and removing precipitated impurities and growth hormone aggregates. The renatured growth hromone is then purified by ion-exchange chromatography.

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Terre Haute, IN, United States of America

Ren-der Yang