Centre National De La Recherche Scientifique

Ecole Polytechnique

Stilla Technologies

Centre National De La Recherche Scientifique-cnrs

Rémi Dangla

Charles Baroud

Paul Abbyad

Étienne Fradet

Silvan Turkcan

Nicolas Fernandez

Barbara André

Jordan Madic

Allison Mallory

Francois Gallaire

Gwilherm Jaspard

ÉTienne Fradet

François Gallaire

The invention relates to an assembly for contactless pressure-controlled release of a fluid comprising a non-compressible compartment, at least two fluids in fluidic contact and enclosed inside the non-compressible compartment, one of the two fluids being compressible, and one channel for fluid flow.

Assembly for pressure controlled fluid release and its method therefore

The subject matter of the present invention is a microfluidic process for treating and analysing a solution containing a biological material, comprising a step of introducing the solution into microchannels of a microfluidic circuit (), a step of forming drops of this solution, under the effect of modifications of the surface tension of the solution, a step of moving the drops to one or more drop storage zones(s) (), under the effect of modifications of the surface tension of the drops, a step of treating the drops and a step of analysing the drops.

Microfluidic process for treating and analysing a solution containing a biological material and corresponding microfluidic circuit

The present invention relates to an emulsion, preferably a water-in-oil emulsion. comprising: a continuous phase comprising a conductivity improving compound, a dispersed phase suspended in the continuous phase, and a surfactant. The present invention also relates to a population of droplets comprising an aqueous phase, dispersed in a continuous oily phase comprising an oil and a conductivity-improving compound. The present invention also relates to a microfluidic chip comprising a hydrophobic composition comprising an oil, a surfactant and a conductivity improving compound in an injection chamber configured so that injecting a hydroplilic composition through said injection means will generate an emulsion in the injection chamber; and to a kit comprising a microfluidic chip and a container comprising an aqueous composition. The present invention also relates to a process for manufacturing an emulsion according to the invention. The present invention also provides methods for analyzing biological material, for example for analyzing biological material within the emulsion of the invention.

Emulsions with improved stability

The present invention relates to a contact-less priming system for loading a solution in a microfluidic device comprising: at least one microfluidic device, a pressure chamber configured to enclose said at least one microfluidic device, a pressurization unit fluidly connected to the pressure chamber and at least one closing member. The present invention also relates to a contact-less priming method for loading a solution in a microfluidic device.

Contact-less priming method for loading a solution in a microfluidic device and associated system

The present application provides multiplex drop-off digital polymerase chain reaction (dPCR) assays, methods, systems, and kits. The methods described herein are useful in a variety of applications, such as detection of microsatellite instability and quantification of site-specific genome-edited products.

Multiplex drop-off digital polymerase chain reaction methods

The subject of the present invention is a microfluidic circuit in which are defined microchannels able to contain fluids and including at least one device for forming drops of a solution, guiding the drops to a storage zone in which one of the drops can be brought into contact with a drop of another solution, the walls of the microchannel portion forming the first drop-formation device diverging so as to cause drops of the first solution to detach under the effect of the surface tension of the first solution; the first guide include wall portions of the microchannels that diverge so as to cause the drops to move along under the effect of the surface tension of the first solution.

Microfluidic circuit allowing drops of several fluids to be brought into contact, and corresponding microfluidic method

Provided herein are microfluidic circuits that include at least one device for forming a quantity of drops of a solution in a carrier fluid and at least one storage zone for storing drops produced by the microfluidic device. Such microfluidic circuits are useful, for example, for the analysis of a solution containing a biological sample.

The invention relates to a microfluidic circuit including at least one microchannel for the flow of a first fluid conveying drops or bubbles of at least one second fluid, characterized in that the height of the microchannel is sized so as to crush the drops or bubbles during the movement thereof, and in that the microchannel comprises at least one trough, extending at least partially in the direction of flow of the first fluid or an area for trapping drops or bubbles, said area or the trough having a height that is greater than the height of the microchannel, such that at least some of the drops or bubbles of the second fluid in the microchannel are drawn and guided into the trough or into the trapping area.

Microfluidic circuit

The invention relates to a device () for forming droplets in a microfluidic circuit, the device comprising a chamber () containing a first fluid and defined by two opposite walls () that diverge relative to each other in at least one given direction, and a microchannel () containing a second fluid and leading into a zone of said chamber () that is upstream relative to the given direction, the outlet of the microchannel () into the chamber () constituting an enlargement in the flow section for the second fluid, and the enlargement giving rise to droplets () of the second fluid forming within the first fluid.

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Paris, France

Rémi Dangla