Sequenom, Inc.

Agilent Technologies, Inc.

Stratagene California

Reinhold Mueller

Charles Cantor

Mathias Ehrich

Grace DeSantis

Joseph A Sorge

Gothami Padmabandu

Holly Hogrefe

Nick Roelofs

Technology provided herein relates in part to methods, processes and apparatuses for non-invasive assessment of genetic variations.

Methods and processes for non-invasive assessment of genetic variations

The invention relates to the generation and characterization of Archaeal DNA polymerase mutants with deficient 3'-5&#x2032; exonuclease activity and reduced base analog detection activity. The invention further provides for Archaeal DNA polymerase mutants with deficient 3&#x2032;-5&#x2032; exonuclease activity and reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or reverse transcriptase activity. The invention also discloses methods and applications of DNA polymerases with deficient 3&#x2032;-5&#x2032; exonuclease activity and reduced base analog detection activity.

DNA polymerase compositions for quantitative PCR and methods thereof

The present invention relates to probes useful for the detection and measurement of target nucleic acids, as well as compositions and kits containing such probes. The probes of the present invention include an interactive pair of labels, as well as a hairpin sequence that does not hybridize to a target nucleic acid. According to the present invention, the probe generates a detectable signal indicative of a presence of a target nucleic acid. The detectable signal emitted by one of the pair of labels is substantially constant upon binding of the probe to target nucleic acid, and the detectable signal increases by at least 2 fold upon cleavage of the probe between the pair of labels.

Dual labeled fluorescent probes

Growing community of inventors

San Diego, CA, United States of America

Reinhold Mueller