Hoechst Aktiengesellschaft

Aventis Pharma Deutschland, Gmbh

Sanofi-a Ventis Deutschland Gmbh

Cassella Aktiengesellschaft

Hoechst Marion Roussel Deutschland Gmbh

Reinhold Keller

Merten Schlingmann

Gerd Fulling

Wolfgang Holla

Franz-Josef Rubr&ouml;der

Lothar Willms

Karl-Heinz Keil

Dieter Wullbrandt

Fritz Engelhardt

Klaus Kuhlein

Manfred Schneider

Joachim Thiem

Ulrich Greiner

Martin Platen

Wolfgang Treder

Friedrich Engelhardt

Gunter Siegemund

Erwin Schmidt

Gerhard Wohner

Hartmut Voelskow

Johann Then

Richard Bicker

Werner Sievers

Matthias Wiesner

Frank Richard

Franz-Josef Rubröder

Kurt Laumen

Merten Schilingmann

Gerhard Hess

Franz-Josef Rubroeder

Dieter Desch

Detlef Breitgoff

Heike Herbert

J&ouml;rg Von Eysmondt

The present invention comprises a process for preparing insulin or an insulin derivative with correctly linked cysteine bridges from a precursor of said insulin or insulin derivative, wherein said precursor is subjected to a folding process in the presence of cysteine or cysteine hydrochloride and a chaotropic auxiliary compound. The insulin or insulin derivative with correctly linked cysteine bridges is obtained by enzymic cleavage by means of trypsin or a trypsin-like enzyme and, where appropriate, additionally by means of carboxypeptidase B and subsequent purification on an adsorber resin, which process is carried out at varied pH and temperature ranges.

Process for the preparation of insulin or an insulin derivative in the presence of oxygen

The present invention relates to a process for the storage of proteins in an aqueous solution. The addition of cysteine delays the temporal decrease in the effective concentration of the protein. The process is suitable for use in the production of heterologous proteins in microorganisms.

Process for the stabilization of proteins in an aqueous solution comprising cysteine in a concentration between 150 and 220mM

Process for the stabilization of proteins in complex mixtures during their storage in aqueous solvents

The present invention relates to an improved process for obtaining a precursor of an insulin or insulin derivative having correctly bonded cystine bridges in the presence of cysteine or cysteine hydrochloride and chaotropic auxiliary.

Process for obtaining insulin precursors having correctly bonded cystine bridges

The present invention relates to an improved procedure for the chromatographic purification of insulins wherein a pressure-stable organic polymeric chromatography material is used as a stationary phase, and the mobile phase contains at least one water-miscible organic solvent and at least one buffer substance and the pH is from about 7 to about 11.

Procedure for the chromatographic purification of insulins

Process for obtaining insulin precursors having correctly bonded cystine

L-PTC (L-phosphinothricin, L-2-amino-4-methylphosphino-butyric acid) is the active herbicidal component of D,L-PTC and can be obtained according to the invention when D,L-PTC derivatives which are N-acylated and esterified on the phosphinic acid group as well as optionally esterified or amidated on the carboxylic group, are treated with a hydrolytically active enzyme in an aqueous or aqueous-organic medium, in which process the L-PTC derivatives are selectively hydrolyzed on the N-acyl group or the modified carboxyl group, the resulting product mixture is resolved, and the desired L-PTC derivative is hydrolyzed to give the L-PTC and isolated by customary methods.

Process for the enzymatic resolution of

Process for the enzymatic resolution of 2-amino-4-methylphosphinobutyric acid derivatives. L-PTC (L-phosphinothricin, L-2-amino-4-methylphosphinobutyric acid) is the active herbicidal component of D,L-PTC and can be obtained according to the invention when D,L-PTC derivatives which are N-acylated and esterified on the phosphinic acid group as well as optionally esterified or amidated on the carboxylic group, are treated with a hydrolytically active enzyme in an aqueous or aqueous-organic medium, in which process the L-PTC derivatives are selectively hydrolyzed on the N-acyl group or the modified carboxyl group, the resulting product mixture is resolved, and the desired L-PTC derivative is hydrolyzed to give the L-PTC and isolated by customary methods.

A process for highly regioselective esterification and ester cleavage on unsaturated sugar compounds with the aid of lipases and esterases, and products which can be prepared by this process. Highly regioselective esterifications and ester cleavages can be carried out on unsaturated sugar compounds with the aid of lipases and esterases. Lipases from microorganisms or from the pancreas and liver of animals are preferably used.

Unsaturatd sugar compounds

In the enzymatic stereoselective ester cleavages of 2-arylpropionate, the reaction rate of the hydrolyzing enzymes can be drastically increased if the vinyl ester of the 2-arylpropionate is employed as the substrate.

Vinyl 2-aryl-propionates

Process for highly regioselective esterification and ester cleavage on

Process for the enzymatic cleavage of vinyl 2-aryl-propionates

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Bad Soden, Germany

Reinhold Keller