Boehringer Mannheim Gmbh

Reinhard Herz

Helmut Lill

Andreas Dessauer

Enno Adema

Ulrike Gebert

In a method for the determination of coagulation parameters in sample material via a reaction cascade in which a thrombin-catalyzed formation of a fibrin clot from fibrin monomers occurs and the formation of the fibrin clot is measured, an inhibitor of F XIII is added. By this means the reaction vessel can be used several times for coagulation tests.

Method for the determination of coagulation parameters

In order to determine antithrombin III in body fluids by reacting the sample with thrombin and a chromogenic substrate which forms a colour by the action of thrombin and measurement of the colour formed, the reaction is carried out in the presence of denaturing agents or of the tetrapeptide Gly-Pro-Arg-Pro and an oligopeptide is used as the substrate which has a sensitivity to thrombin which is a factor of 5 to 100-fold lower compared With Tos-Gly-Pro-Arg-pNA

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Munich, Georgia

Reinhard Herz