Aventis Pasteur S.a.

Pasteur Merieux Serums Et Vaccins

Pasteur Merieux Serums & Vaccins

Regis Sodoyer

Luc Aujame

Frederique Geoffroy

Annabelle Bouchardon

Michel Chevalier

Robert Brasseur

Tino Krell

Benoit Charloteaux

Raphaelle El Habib

The present invention thus provides a polypeptide capable of forming a structure corresponding to or mimicking the intermediate of gp41 as well as its use in a vaccine for treating or preventing HIV infections.

Polypeptide inducing antibodies neutralizing HIV

On the basis of a first repertoire of genes coding for a population of one of two kinds of polypeptides capable of being combined, particularly an antibody light chain variable region, and at least one gene, and preferably a second repertoire coding for the other kind of polypeptide, particularly an antibody heavy chain variable region, the genes from the first repertoire are inserted into a first vector to form a vector population and the genes from the second repertoire are inserted into a second vector, at least one of said vectors being a recipient for the expression of both genes as polypeptides irreversibly combined with the outer surface of the product of said vector.

Process for preparing a multicombinatorial library of vectors for expressing antibody genes

On the basis of a first repertoire of genes coding for a population of one of two kinds of polypeptides capable of being optionally covalently combined, particularly variable regions of either the antibody light chain type or the antibody heavy chain type, and at least one gene coding for the other type of polypeptide, particularly a variable region of the other type, an antibody chain or preferably a second repertoire of genes coding for a population of said other type, the genes from the first repertoire are inserted into a first vector to form a population of vectors carrying the various genes of said first repertoire, and said gene of said other type or the genes from said second repertoire is/are inserted into a second vector. Both starting vectors have means enabling each to exchange one part by one or more irreversible recombinations to generate recombinant final vectors of which one contains a gene of one of said types and a gene of the other type.

Preparation of a multicombinatorial library of antibody gene expression vectors

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Sainte Foy Les Lyon, France

Regis Sodoyer