Centre National De La Recherche Scientifique

Université Technologie De Compiègne-utc

Ecole Normale Superieure De Lyon

Institut National De L'environment Industriel Et Des Risques

Institut National De L' Environnement Industriel Et Des Risques

Régis Baudoin

Eric Leclerc

Cécile Legallais

Patrick Paullier

Marc-Emmanuel Dumas

Céline Brochot

Laetitia Shintu

Jean-Matthieu Prot

Pierre Toulhoat

The present invention relates to a unit () comprising a microsystem () and at least one set of interface connections () for the microsystem (); the microsystem () comprising a bottom plate () bearing the impression of at least one microstructured dynamic cell culture chamber (), and a top plate (), characterized in that the at least one microstructured chamber () is fluidically connected at inlet and/or at outlet by a set of connections () inserted removably into a hole in the upper plate (). The present invention also relates to a dynamic cell culture system to this effect.

Multi-reactor unit for dynamic cell culture

The present invention relates to a method of toxicological evaluation of a candidate substance on at least one tissue or organ, characterized in that it comprises the steps of: (a) obtaining a bioartificial tissue or organ by simulation or modelling of the metabolic activity of said tissue or organ by at least one bioreactor; (b) exposure of said bioartificial tissue or organ to said substance; (c) observation of the metabolic response of the bioartificial tissue or organ and acquisition without a priori of an associated multidimensional data set; (d) identification by means of a method of multivariate statistical analysis of the components of the multidimensional data set which are quantitatively correlated with predetermined variables; (e) generation of a predictive model on the basis of the components of the data set that are actually retained; (f) testing of the predictive nature of said model by at least one statistical method of estimating reliability; (g) identification of the metabolic response of the bioartificial tissue or organ in the form of biomarkers associated with the components of the data set that are adopted for the model. The present invention also relates to a method of toxicological screening and to a system for this purpose.

Method of toxicological evaluation, method of toxicological screening and associated system

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Neuilly Plaisance, France

Régis Baudoin