Novozymes, Inc.

Novozymes A/s

Novozymes Biopharma Dk A/s

Regine Behr

Alan P Sloma

Maria Tang

David Sternberg

Stephen H Brown

William R Widner

Linda Sternberg, Legal Representative

Leslie Naggiar, Legal Representative

Carsten Schau Andersen

Shiro Fukuyama

Anders Viksoe-Nielsen

Stephen Brown

William Widner

The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating ahost cell under conditions suitable for production of the hyaluronic acid, wherein thehost cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase.

Methods for producing hyaluronan in a recombinant host cell

The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parentcell under conditions conducive for the production of the heterologous biological substance, wherein (i) the mutant cell comprises a first nucleic acid sequence directing synthesis of the heterologous biological substance and a second nucleic acid sequence comprising a modification of at least one of the genes cypX and yvmC, which are involved in the production of a red pigment, and (ii) the mutant cell is deficient in the production of the red pigment compared to the parentcell when cultivated under the same conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to mutants ofcells and methods for producing the mutants.

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Polypeptides comprising a catalytic starch debranching domain and a starch binding domain are disclosed. The polypeptides comprising the starch binding domain have an improved functionality in raw starch degradation compared with same catalytic unit in same amount but without the starch binding domain.

Polypeptides with starch debranching activity

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Roseville, CA, United States of America

Regine Behr