Synthego Corporation

Bionex Solutions, Inc.

Reed Kelso

Travis Maures

John Andrew Walker, Ii

David K Matsumoto

Eric J Rollins

Benjamin N Shamah

Jared Carlson-Stevermer

Anastasia Kadina

Sahil Joshi

Mark R Sibenac

Paul Dabrowski

Fabian Gerlinghaus

Tony Lima

Antonio M Lima

The present invention provides improved automated systems and methods for synthesis of biopolymers including DNA and RNA. The automated systems and methods represent a number of improvements over existing systems for multiplex synthesis of biopolymers in a combinatorial fashion.

Guide RNA synthesis system and method

Provided herein are polynucleotides and CRISPR effector proteins configured to be covalently bound together in a CRISPR complex. The polynucleotides can be further modified to modulate the activity of the CRISPR complex. Modification of the polynucleotide and CRISPR effector protein can be used to improve the efficacy of target binding and/or cleavage.

Stabilized CRISPR complexes

Methods and systems for preparing clonal cell populations are described. In some instances the disclosed methods comprise: a) identifying and selecting a cell based on its position on a surface or in a container, where the selection is not based on whether the cell comprises an exogenous label or an expressed reporter; b) photoablating all non-selected cells on the surface or in the container; and c) growing a clonal population of the selected cell.

Cell culture laser photoablation

Provided herein are polynucleotides comprising sequence configured to bind to a CRISPR effector protein. Modulation of one or more modifications of the polynucleotides can be used to tune the activity of CRISPR effector proteins complexed with the polynucleotides.

Systems and methods for modulating CRISPR activity

Material transfer/interrogation devices (e.g. liquid handling workstations) have been designed in the past for transferring material from a source to a destination location or for interrogating a material at a location, where the locations remain fixed. The invention provides methods and apparatuses for transferring or interrogating materials by one or more carrier devices to one or more receiving devices, where the carrier and receiving devices move independently and simultaneously on multiple axes. In some embodiments, one or more of the carrier and receiving devices can move along an X, Z, Y, and Theta axis, which allows the source and destination locations to rotate and translate relative to each other. Due to this rotation and translation, containers can be positioned to minimize the distance traveled between a pick location from the source and a place location on the destination, greatly increasing the speed at which material transfer can occur.

Transfer or interrogation of materials by carrier and receiving devices moving independently and simultaneously on multiple axes

Growing community of inventors

San Francisco, CA, United States of America

Reed Kelso