Centre National De La Recherche Scientifique

Institut Pasteur De Lille

Université De Lille 1 - Sciences Et Technologies

Reda Mhidia

Oleg Melnyk

Nathalie Ollivier

Julien Dheur

Method for preparing a peptide assembly of n fragments and n&#x2212;1 amino acids bearing a thiol function, represented by the formula:A-C-A-C-A- . . . -C-A- . . . -C-A&#x2003;&#x2003;(I)in which A, A, A, . . . A. . . , Aare peptide fragments, C, C, C. . . C. . . Care amino acid residues bearing a thiol function, n is comprised between 3 and 50, and i is 2 to n, in which a peptide-thioester is prepared of formula: A-SR (II) in which Ais a peptide fragment and SR is an alkyl thioester residue, R being alkyl optionally substituted, starting from a bis(2-sulphanylethyl)amino peptide.

Method for preparing peptides by assembling multiple peptide fragments

The invention mainly relates to a method for manufacturing a polypeptide of formula:X&#x2014;X&#x2033;&#x2014;X&#x2003;&#x2003;(III)Xand Xeach representing a peptide fragment, and X&#x2033; representing an amino acid residue comprising a thiol function, said method comprising at least one step of ligation reaction between a polypeptide of formula:X&#x2014;N(CHCHSH)&#x2003;&#x2003;(I)and a polypeptide of formula:H&#x2014;X&#x2033;&#x2014;X.&#x2003;&#x2003;(II) The invention also relates to the polypeptides of formula (I) themselves and the method for obtaining them, as well as resin supports suitable for obtaining them.

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Reda Mhidia