Allergan, Inc.

Silk Therapeutics, Inc.

Tufts University

Evolved by Nature, Inc.

Tissue Regeneration, Inc.

Rebecca L Horan

Gregory H Altman

Jingsong Chen

Adam L Collette

Dylan S Haas

David J Horan

Rachel M Lind

Rachel Lee Dow

Dennis E Van Epps

Wha Bin Im

Jingson Chen

David L Kaplan

Wha-Bin Im

Guang-Liang Jiang

Jing Song Chen

David Horan

A composition is disclosed that includes pure silk fibroin-based protein fragments that are substantially devoid of sericin, wherein the composition has an average weight average molecular weight ranging from about 17 kDa to about 38 kDa, wherein the composition has a polydispersity of between about 1.5 and about 3.0, wherein the composition is substantially homogeneous, wherein the composition between 0 ppm to about 500 ppm of inorganic residuals, and wherein the composition includes between 0 ppm to about 500 ppm of organic residuals.

Stable silk fibroin based pharmaceutical formulations

Stable silk protein fragment compositions

The present specification provides for methods for purifying fibroins, purified fibroins, methods of conjugating biological and synthetic molecules to fibroins, fibroins conjugated to such molecules, methods of making fibroin hydrogels, fibroin hydrogels and fibroin hydrogel formulations useful for a variety of medical uses, including, without limitation uses as bulking agents, tissue space fillers, templates for tissue reconstruction or regeneration, cell culture scaffolds for tissue engineering and for disease models, surface coating to improve medical device function, or drug delivery devices.

Silk fibroin hydrogels and uses thereof

Silk is purified to eliminate immunogenic components (particularly sericin) and is used to form fabric that is used to form tissue-supporting prosthetic devices for implantation. The fabrics can carry functional groups, drugs, and other biological reagents. Applications include hernia repair, tissue wall reconstruction, and organ support, such as bladder slings. The silk fibers are arranged in parallel and, optionally, intertwined (e.g., twisted) to form a construct; sericin may be extracted at any point during the formation of the fabric, leaving a construct of silk fibroin fibers having excellent tensile strength and other mechanical properties.

Sericin extracted fabrics

The specification discloses compositions and methods for treating a soft tissue defect of an individual.

Compositions and soft tissue replacement methods

The invention relates to compositions comprising a silk fibroin material, adipose tissue and a compound of the structure where Xand Xare independently CH or N; Xis NH, O or S; Xand Xare independently CH or N; Ris an optionally substituted aryl or optionally substituted heteroaryl and Ris Calkyl, optionally substituted aryl or optionally substituted heteroaryl. The composition is administered to a site of a soft tissue condition including those of breast tissue. The silk fibroin material can be in the form of a silk hydrogel, a silk film, a silk scaffold, a silk sheet, a silk porous material or a silk fiber. The silk fibroin material can be substantially sericin free.

The invention relates to compositions comprising adipose tissue, a silk fibroin material and a compound of the structure The composition is administered to a site of a soft tissue condition including those of breast tissue. The silk fibroin material can be in the form of a silk hydrogel, a silk film, a silk scaffold, a silk sheet, a silk porous material, a silk fiber or a combination of those materials. The silk material may be substantially sericin free.

Growing community of inventors

Arlington, MA, United States of America

Rebecca L Horan