Amgen Inc.

Immunex Corporation

Rebecca E McCoy

Arvia Eleanor Morris

Brian D Follstad

Helmut M Sassenfeld

Jian Wu

Richard L Remmele, Jr

Shun Luo

Henry Lin

Matthew I Jerums

Amanda Kano

Leslie Phillip Miranda

Sohye Kang

Rohini Deshpande

The invention provides a method for culturing mammalian cells. The method provides greater control over cell o growth to achieve high product titer cell cultures.

Mammalian cell culture

The invention provides a method for culturing mammalian cells. The method provides greater control over cell growth to achieve high product titer cell cultures.

The invention provides a method for culturing mammalian cells. The method provides greater control over cell growth to achieve high product titer cell cultures by changing the temperature of the cell culture and/or by starving the cells in their asparagine supply.

The present invention relates to methods for mammalian cell culture. The methods make use of independent tyrosine and cystine feed streams.

Method for culturing mammalian cells to improve recombinant protein production

The present invention relates to the culture of animal cells in serum-free culture medium. The present invention provides particular dipeptides that can improve recombinant protein production and cell viability in such cultures, especially in the absence of peptones.

Dipeptides to enhance yield and viability from cell cultures

The invention provides methods of increasing yields of desired conformation of proteins. In particular embodiments, the invention includes contacting preparations of a recombinant protein with a reduction/oxidation coupling reagent for a time sufficient to increase the relative proportion of a desired configurational isomer.

Increased recovery of active proteins

The present invention provides polypeptide purification reagents that are, themselves, proteins and methods for using these to purify almost any protein of interest. A polypeptide purification reagent comprises a polypeptide domain that can bind to a protein of interest and a polypeptide domain that confers a propensity to precipitate under conditions where the vast majority of proteins will not precipitate. In addition or as an alternative, a polypeptide purification reagent can comprise amino acid sequences that confer an ability to partition into a phase in a liquid&#x2014;liquid phase system that the majority of proteins do not partition into. Methods for purifying a protein of interest using such polypeptide purification reagents are also provided. The methods include forming a precipitate comprising the polypeptide purification reagent and the protein of interest under conditions in which the vast majority of proteins do not precipitate and in which the biological activities of both proteins are substantially preserved. The methods further include an optional repurification by one or more reprecipitation steps or by affinity chromatography. In addition or as an alternative, the methods include recovering the protein of interest bound to the polypeptide purification reagent in a phase of a liquid&#x2014;liquid phase system. The methods further include separating the protein of interest from the polypeptide purification reagent.

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Seattle, WA, United States of America

Rebecca E McCoy