Akonni Biosystems, Inc.

Ionian Technologies, Inc.

Rebecca C Holmberg

Phillip Belgrader

Jeffrey G Mandell

Richard B Roth

Andrew P Miller

Jarrod Provins

Brian K Maples

Christopher G Cooney

Peter Qiang Qu

Tinsley Janna Stokes

Alissa Erin Burkey

Alissa Erin Gindlesperger

David E Sipes

An integrated sample purification system includes a housing, a sample container rack, a filter holder, and a cylindrical magnet. The sample container rack and the filter device holder are disposed in the housing. The sample container rack is configured to hold one or more sample containers, the filter device holder is configured to hold one or more filter devices. The cylindrical magnet is adjacent to and external to the sample container rack, and is rotated about a central, longitudinal axis of the magnet by an electric motor disposed in the housing to lyse cells. Molecules of interest in the lysed cells are purified using filters that bind specifically to the molecules of interest. The system is readily amenable to automation and rapid purification and analysis of molecules of interest, such as nucleic acids and proteins.

Integrated sample processing system

The invention is in general directed to the rapid exponential amplification of short DNA or RNA sequences at a constant temperature.

Nicking and extension amplification reaction for the exponential amplification of nucleic acids

Methods for automated extraction of nucleic acids are disclosed. Also disclosed are method and kits for isolating fetal nucleic acids from a plasma sample of a pregnant woman. The method includes flowing the plasma sample through a first filter under conditions that allow binding of the fetal and maternal nucleic acids to the first filter; eluting the fetal and maternal nucleic acids bound to the first filter to produce a concentrated nucleic acid sample; flowing the concentrated nucleic acid sample through a second filter under conditions that allow preferential binding of the maternal nucleic acids to the second filter; and recovering the fetal nucleic acid from the concentrated nucleic acid sample that flow through the second filter.

Method and kit for purifying nucleic acids

A microarray system is disclosed. The microarray system includes a microarray formed on a planar substrate and an incubation chamber formed around the microarray. The incubation chamber has a plurality of interior surfaces including a bottom surface on which the microarray is formed and a top surface that faces the bottom surface and is generally parallel to the bottom surface. At least one of a plurality of interior surfaces is a hydrophilic surface.

Flow cell device

Growing community of inventors

San Diego, CA, United States of America

Rebecca C Holmberg