California Institute of Technology

Lonza Sales Ag

Raymond W Bourdeau

Mikhail G Shapiro

Arash Farhadi

Anupama Lakshmanan

Suchita P Nety

Michael Doherty

Audrey Lee-Gosselin

Aaron R Noyes

Konstantin Konstantinov

Douglas E Williams

Kathryn E Golden

Michael P Mercaldi

Kimberly Ellis

Kayla Desanty

Gas vesicles, protein variants and related compositions methods and systems for singleplexed and/or multiplexed ultrasound imaging of a target site in which a gas vesicle provides contrast for the imaging which is modifiable by application of a selectable acoustic collapse pressure value of the gas vesicle.

Gas-filled structures and related compositions, methods and systems to image a target site

Methods of loading extracellular vesicles with payload molecules via homogenization are disclosed herein.

Loading of extracellular vesicles through imparting of mechanical shear

The present disclosure relates to multistep chromatographic methods for preparing extracellular vesicles (EVs). The methods were demonstrated to be effective in preparing highquality EVs in a large scale. The methods enable preparation of EVs for therapeutic and diagnostic applications, and isolation and/or sub-fractionation of EVs with desired properties for specific use.

Process for preparing extracellular vesicles

Hybrid gas vesicle gene cluster (GVGC) configured for expression in a prokaryotic host are described comprising gas vesicle assembly (GVA) genes native to a GVA prokaryotic species and capable of being expressed in a functional form in the prokaryotic host, and one or more gas vesicle structural (GVS) genes native to one or more GVS prokaryotic species, at least one of the one or more GVS prokaryotic species different from the GVA prokaryotic species, and related gas vesicle reporting (GVR) genetic circuits, genetic, vectors, engineered cells, and related compositions methods and systems to produce GVs, hybrid GVGC and/or image a target site.

Genetically engineered gas vesicle gene clusters, genetic circuits, vectors, prokaryotic cells, compositions, methods and systems for contrast-enhanced imaging

Growing community of inventors

Watertown, MA, United States of America

Raymond W Bourdeau