Cryopharm

Baxter International Inc.

Cobe Laboratories, Inc.

California Institute of Technology

Credit Managers Association of California

Raymond P Goodrich, Jr

Matthew Stewart Platz

Christine M Williams

Nagender Yerram

Samuel O Sowemimo-Coker

Roger W Hackett

Sang Chul Park

Brian M Aquila

John D Baldeschwieler

Francoise Arnaud

Samuel O Coker

Victoria A Wong

Murray Weiner

Robert S Franco

Vijay Adda

Johannes Derksen

Iris Williams

Robert Hudak

Gonzalo M Cabrera

Richard F Galle

Miller Cho

Jon A Olson

Nagendar Yerram

Marjan Van Borssum Waalkes

Christine M Williams-Hughes

A method is presented for inactivating non-enveloped viruses that may be contaminating a biological solution or suspension by mixing the solution or suspension with a photosensitizer to form a mixture, adjusting the operating conditions of the mixture so as to increase the permeability of the viruses to the photosensitizer, and then irradiating the adjusted mixture.

Method for inactivating non-enveloped viral contaminants with a photosensitizer by increasing viral permeability to the photosensitizer

Viral and bacterial contaminants present in biological solutions are inactivated by mixing one of a novel class of photosensitizer with said solution and irradiating the mixture. Quinoline and quinolone compounds are useful as photosensitizers in this method. With certain photosensitizers a blocking agent may also be employed.

Method of inactivation of viral and bacterial blood contaminants

A method is provided for inactivating viral and/or bacterial contamination in blood cellular matter, such as erythrocytes and platelets, or protein fractions. The cells or protein fractions are mixed with chemical sensitizers, frozen or freeze-dried, and irradiated with, for example, UV, visible, gamma or X-ray radiation while in the solid state.

Viral and bacterial contaminants present in biological solutions are inactivated by mixing one of a novel class of photosensitizer with said solution and irradiating the mixture.

A process is provided for freezing, including freeze-drying of cells, cell-membranes or cell-like materials using a cryoprotectant medium which stabilizes the cells or membranes for freezing or freeze-drying and allows for freezing or freeze-drying to be performed at -60.degree. C. or higher.

Method of freezing cells and cell-like materials

Viral, bacterial and parasitic contaminants in blood and cell culture containing compositions are inactivated by adding a photosensitizer and a blocking agent to the composition and irradiating the resulting mixture with electromagnetic radiation. Blocking agents reduce photolysis of the photosensitizer by mechanisms not involving the quenching of reactive oxygen species. The blocking agents reduce photolysis which occurs by ring opening. Preferred photosensitizers are quinoline or quinolone.

A method is provided for the lyophilization of mammalian sperm cells which can be stored and reconstituted to provide morphologically intact cells. The method is advantageous in that the sperm cells may be stored at ambient temperatures for extended periods of time and recovered morphologically intact with DNA-containing heads and intact flagella. The lyophilization medium comprises a carbohydrate and a polymer selected from PVP, HES, dextran and poloxamers or mixtures thereof.

Method of lyophilization of mammalian sperm cells

Method of inactivation of viral and bacterial blood contaminants with

Method of detecting circulating antibody types using dried or

A process and medium are disclosed for the lyophilization of cells which comprises the use of solutions including monosaccharide hexoses and pentoses, and/or biocompatible amphipathic polymers to permit the reconstitution of viable cells.

Lyophilization of cells

A method is disclosed for preparing lyophilized and frozen cells as cytometry standards.

Method of preparing lyophilized and frozen cell standards

Growing community of inventors

Pasadena, CA, United States of America

Raymond P Goodrich, Jr