Vestar, Inc.

Gilead Sciences, Inc.

City of Hope

Raymond L Teplitz

Richard T Proffitt

George W Tin

Lawrence E Williams

Cary A Presant

Lloyd H Smith

Micellular particles such as small unilamellar vesicles of less than 2000 A&deg; loaded with,In are administered to BALB/c mice in which EMT,tumors had been induced. Whole body scintographs of the mice to which either neutral or positively or negatively charged vesicles had been administered show a substantial quantity of the vesicle entrapped,In localized in the tumor. Blocking of macrophages in the liver and spleen by first administering unlabeled, aminomannose substituted vesicles before administration of the labeled vesicles increases uptake of the,In labeled vesicles in the tumor.

Method of targeting a specific location in a body using phospholipid and cholesterol liposomes which encapsulate an agent

A method is provided for delivering micellular particles containing chemotherapeutic agents and a marker to tumors within a body for the diagnosis and treatment of such tumors. The micellular particles are small, less than 2000 .ANG. and incorporate pure, neutral phospholipid molecules in their external surface. Enhanced delivery of the micellular particles containing marker and chemotherapeutic agents may be achieved by introducing an initial group of positively charged micellular particles to block the reticuloendothelial cells present in the body.

Method of delivering micellular particles encapsulating chemotherapeutic

Micellular particles such as small unilamellar vesicles of less than 2000 .ANG. loaded with .sup.111 In are administered to BALB/c mice in which EMT6 tumors had been induced. Whole body scintographs of the mice to which either neutral or positively or negatively charged vesicles had been administered show a substantial quantity of the vesicle entrapped .sup.111 In localized in the tumor. Blocking of macrophages in the liver and spleen by first administering unlabeled, aminomannose substituted vesicles before administration of the labeled vesicles increases uptake of the .sup.111 In labeled vesicles in the tumor.

Method of targeting a specific location in a body

A method is provided for testing for particular antibodies in the serum of a patient. The antibodies may be those of systemic lupus erythematosus and may constitute IgG and IgM immunoglobulins. The IgG and IgM immunoglobulin may be individually labeled radioactively. An antigen (such as DNA) may be attached to a support such as sepharose. The attachment may be facilitated as by irradiation with ultraviolet light. The DNA may be single stranded or double stranded. When double-stranded DNA is used, single-stranded portions in the double strands may be removed as by a suitable enzyme. The particular antibodies may be attached to the antigen such as the supported DNA. An assay may then be provided to determine the attachment of the particular antibodies to the supported DNA. When the particular antibodies constitute immunoglobins of systemic lupus erythematosus, the assay may actually provide a determination of the amounts of the IgG and IgM immunoglobulins individually attached to the single-stranded and double-stranded DNA.

Growing community of inventors

Pasadena, CA, United States of America

Raymond L Teplitz