Institut Pasteur

Institut National De La Recherche Agronomique

Other

Centre National De La Recherche Scientifique

Institut Pasteur and Institute National De La Rocherche Agronomic

Raymond Dedonder

Didier Lereclus

Vincent Sanchis

Ghislaine Menou

Marguerite-Marie Lecadet

Daniel Martouret

Andre Klier

Georges Rapoport

Frederik Kunst

Antoine P Amory

This invention relates to vectors, bacterial strains, and methods for the cloning and expression of a polypeptide having larvicidal activity. In particular, the invention relates to vectors, bacterial strains and methods for the cloning and expression of the N-terminal region of a polypeptide toxic against the larvae of Lepidoptera of the Noctuidae family, preferably against

Nucleotide sequences coding for polypeptides endowed with a larvicidal activity towards Lepidoptera

This invention relates to a method for the cloning a polypeptide having larvicidal activity. In particular, the invention relates to vectors, bacterial strains and methods for the cloning and expression of the N-terminal region of a polypeptide toxic against the larvae of Lepidoptera of the Noctuidae family, preferably against

Methods for obtaining nucleotide sequences coding for polypeptides specifically active for larvae of S. littoralis

This invention relates to plant cells, plants, and seeds expressing a polypeptide having larvicidal activity. In particular, the invention relates to plant cells, plants, and seeds expressing the N-terminal region of a polypeptide toxic against the larvae of Lepidoptera of the Noctuidae family, and preferably against

Polypeptides endowed with a larvicidal activity toward Lepidoptera

A nucleotide sequence coding for at least one part of the terminal N region of a polypeptide which exercises a specific toxic effect on Lepidoptera of the Noctuidae family, preferably on S.littoralis, is characterized by its capacity for hybridization with a gene capable of expressing a polypeptide exercising a toxic effect on S.littoralis larvae.

Nucleotide sequences coding for polypeptides endowed with a larvicidal

The invention relates to a vector for transforming microorganisms capable of undergoing sporulation and which contains itself a heterologous insert comprising a DNA sequence coding for at least part of crystal protein, particularly that of B. thuringensis. It also concerns the polypeptides expressed by said microorganisms and having insecticidal properties similar to those of crystal protein, the microorganisms themselves, as transformed by this vector and insecticidal composition including either said polypeptide or the microorganism itself.

DNA fragment encoding insecticidal crystal proteins from Bacillus

This invention relates to plant cells, plants, and seeds expressing a polypeptide having larvicidal activity. In particular, the invention relates to plant cells, plants, and seeds expressing the N-terminal region of a polypeptide toxic against the larvae of Lepidoptera of the Noctuidae family, and preferably against S.littoralis.

The invention relates to any fragment of DNA derived from the genome of B. licheniformis coding for a polypeptide of 46 amino acids, or for any fragment of this polypeptide possessing the property of stimulating the production of enzymes secreted by B. subtilis by introduction of the above-mentioned fragment of DNA into this latter by the intermediary of a plasmid. The invention also relates to a procedure for the stimulation of the production of a specific polypeptide by B. subtilis by introduction of the above-mentioned fragment of DNA in a strain of B. subtilis, the genome of which has been modified so that it contains a sequence of DNA coding for the said specific polypeptide.

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Chatenay Malabry, France

Raymond Dedonder