Ciba-geigy Corporation

Raymond D Shillito

Ingo Potrykus

Jerzy Paszkowski

Thomas Hohn

Barbara Hohn

Michael William Saul

Vaclav Mandak

A novel process for the direct transfer of foreign genes to plant genomes is described. The novel process comprises placing a gene under the control of plant expression signals and transferring it, by contact with protoplasts without the aid of natural systems for infecting plants, directly to plant cells from which genetically transformed plants can subsequently be derived.

Transformation of hereditary material of plants

The invention describes a process for culturing proliferating aggregates of plant cells, which comprises (a) uniformly plating isolated protoplasts, or isolated cells regenerated from protoplasts, in or on an agarose-solidified culture medium, and/or (b) cutting this pretreated and solidified culture medium into segments, transferring said segments to a liquid nutrient solution and continuing culturing in both cases until the cell aggregates have attained the desired size.

Propagation process employing agarose medium

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Rheinfelden, Switzerland

Raymond D Shillito