University of California

The Scripps Research Institute

Wisconsin Alumni Research Foundation

Biomarin Pharmaceutical Inc.

Receptos, Inc.

Raymond C Stevens

Earl Cornell

Robert A Nordmeyer

Derek T Yegian

Bernard D Santarsiero

Joseph M Jaklevic

Peter G Schultz

William F Kolbe

Michael Allen Hanson

Gyorgy Snell

Quan Cheng

Vadim Cherezov

Carl W Cork

Thomas N Earnest

Eric Arthur Johnson

Deborah H Charych

Jie Song

Jian Jin

Paul Andrew Fitzpatrick

William Howard Tepp

Daniel I Oppenheimer

Peter Kuhn

Mark T Griffith

Lin Wang

Guangyun Lin

Peter G Shultz

Wei Liu

Joseph M Jaklevich

William H Tepp

Donald C Uber

Arthur L Jones

Veli-Pekka Jaakola

Mark Joseph Jacobson

Pal Erik Gustav Stenmark

Emilio Kakkis

Robert Heft

Alejandra Gamez

Woomi Kim

Mary Straub

Marianne Patch

Vsevolod Katritch

Aaron A Thompson

Joshua M Kunken

Fei Xu

Christopher B Roth

Earl W Cornell

Paul Stenmark

The invention provides the structure of human Aadenosine receptor protein bound to an antagonist. Methods of using one or more binding sites and other features of this G-protein coupled receptor to develop new therapeutics are also disclosed.

[object Object]

A novel subtype of type A botulinum neurotoxin (BoNT/A) is disclosed in the application. Methods to purify the neurotoxin as well as uses thereof are also disclosed.

The invention describes compositions and method useful for the crystallization of membrane proteins.

Methods and compositions for obtaining high-resolution crystals of membrane proteins

The present invention pertains to the use of the protein phenylalanine ammonia-lyase, as well as the biologically-active derivatives of the said protein for preventing or treating diseases associated with a phenylalanine imbalance in a human or animal body. More particularly, the present invention relates to the therapeutic use of the above-cited molecules for preventing or treating a phenylalanine imbalance in vivo. This invention also deals with therapeutic compositions comprising a pharmaceutically active amount of the above-described therapeutic molecules as well as with therapeutic methods using the said therapeutic compositions. Finally, the present invention relates to processes for selecting more therapeutically-effective variants of said protein as well as to the selected variants themselves.

Variants and chemically-modified variants of phenylalanine ammonia-lyase

A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.

Integrated crystal mounting and alignment system for high-throughput biological crystallography

The present invention herein provides the design, synthesis and characterization of compositions comprising asymmetric bolaamphiphilic lipids that form extended polymeric ribbons and wide sheets. These compositions may be doped, or interspersed, with various compounds to fine-tune the fluidity and rigidity of the bolaamphiphilic lipid composition, and promote other morphologies of the composition, including fluid vesicles and truncated flat sheets. Upon an increase in pH these compositions undergo a calorimetric and morphological transformation.

Color and shape changing polymeric ribbons and sheets

A method is provided for performing array microcrystallizations to determine suitable crystallization conditions for a molecule, the method comprising: forming an array of microcrystallizations, each microcrystallization comprising a drop comprising a mother liquor solution whose composition varies within the array and a molecule to be crystallized, the drop having a volume of less than 1 microliter; storing the array of microcrystallizations under conditions suitable for molecule crystals to form in the drops in the array; and detecting molecule crystal formation in the drops by taking images of the drops.

Automated method for setting up multiple crystallization experiments in submicroliter volumes

A method is provided for forming crystallization experiments for a molecule, the method comprising forming a plurality of crystallization experiments that comprise a molecule to be crystallized and a composition that varies among the plurality of crystallization experiments, the crystallization experiments having a volume less than 1 microliter, the volumes of the plurality of crystallization experiments varying among the experiments by less than 25 nL.

Method for performing submicroliter crystallization experiments with high experiment to experiment precision

Method for performing high density submicroliter crystallization experiments

A method is provided for performing crystallization experiments for a molecule, the method comprising performing a plurality of crystallization experiments where the crystallization experiments have volumes of less than 1 microliter, the crystallization experiments comprising a molecule to be crystallized and a composition that varies among the plurality of crystallization experiments detecting crystal formation in the crystallization experiments.

Method for performing submicroliter crystallization experiments

A method is provided for characterizing a crystal structure of a molecule, the method comprising performing a plurality of crystallization experiments where the crystallization experiments have volumes of less than 1 microliter, the crystallization experiments comprising a molecule to be crystallized and a composition that varies among the plurality of crystallization experiments and diffracting a crystal formed in one of the crystallization experiments.

Method for diffracting crystals formed by submicroliter crystallization experiments

Method for screening microcrystallizations for crystal formation

The present invention provides methods and compositions for detecting the presence of biologically-important analytes by using redox liposome biosensors. In particular, the present invention provides liposome/sol-gel electrodes suitable for the detection of a wide variety of organic molecules, including but not limited to bacterial toxins.

Methods for using redox liposome biosensors

A method is provided for performing array microcrystallizations to determine suitable crystallization conditions for a molecule. The method comprises forming an array of microcrystallizations, each microcrystallization comprising a drop containing a molecule to be crystallized, the drop having a volume of less than 1 &mgr;L; storing the array of microcrystallizations under conditions suitable for molecule crystals to form in the drops in the array; and detecting molecule crystal formation in the drops.

Methods and apparatus for performing array microcrystallizations

Growing community of inventors

La Jolla, CA, United States of America

Raymond C Stevens