Beckman Coulter, Inc.

Other

Raymond C Milton

Robert S Matson

A method for the acyl fluoride activation of a carboxysilyl-coated glass is described. The method includes providing a glass substrate having a surface with at least one pendant carboxysilyl linker comprising a carboxyl group and activating without etching the surface of the glass by converting the carboxyl group into an acyl fluoride group. In one embodiment, the surface of the glass may be activated by contacting the surface with a fluoridating agent and a tertiary amine reagent, and a solvent that is capable of binding hydrogen fluoride. A substrate for the covalent immobilization of nucleophile-containing species is also provided. The substrate comprises a glass having a surface with a pendant carboxysilyl linker activated by acyl fluoride without etching the surface of the glass. The invention also provides a bioarray formed on a glass surface activated with acyl fluorides without etching.

Acyl fluoride activation of carboxysilyl-coated glass substrates

Synthesis and use of acyl fluorides of cyanine dyes

A method of attaching unmodified biopolymers, particularly, unmodified polynucleotides, directly to a solid support is provided. The method includes the steps of (a) providing unmodified biopolymers; (b) providing a solid support having at least one surface comprising pendant acyl fluoride functionalities, and (c) contacting the unmodified biopolymers with the solid support under a condition sufficient for allowing the attachment of the biopolymers to the solid support. The unmodified biopolymers may be nucleic acids, polypeptides, proteins, carbohydrates, lipids and analogues thereof. The unmodified polynucleotides may be DNA, RNA or synthesized oligonucleotides. The DNA may be single or double stranded. A device including a solid support and unmodified biopolymers attached to the solid support by reaction with the pendant acyl fluoride functionalities of the solid support is also provided. The methods and devices of the present invention may be used in performing hybridization assays and immunoassays.

Immobilization of unmodified biopolymers to acyl fluoride activated substrates

Reagents for the immobilization of biopolymers, processes for their preparation and their subsequent use in the immobilization of biopolymers for analytical and diagnostic procedures are described. One type of reagent includes a solid support fabricated of a polymeric material having at least one surface with pendant acyl fluoride functionalities. Another reagent includes solid supports fabricated of polymeric materials including ethylene acrylic acid or ethylene methacrylic acid copolymers and activated polypropylene. Processes for preparing reagents include derivatizing polymeric materials to form acyl fluoride functionalities or derivatizing ethylene acrylic acid copolymers and ethylene methacrylic acid copolymers to form active acyl functionalities. Processes for immobilizing biopolymers include attaching natural or presynthesized biopolymers to activated solid support surfaces and directly attaching in a step-wise successive manner biomonomer units to a growing biopolymer chain attached to the solid support reagent.

Polymeric reagents for immobilizing biopolymers

Reagents for the immobilization of biopolymers, processes for their preparation and their subsequent use in the immobilized of biopolymers for analytical and diagnostic procedures are described. One type of reagent includes a solid support fabricated of a polymeric material having at least one surface with pendant acyl fluoride functionalities, Another reagent includes solid supports fabricated of polymeric materials including ethylene acrylic acid or ethylene methacrylic acid copolymers and activated polypropylene. Processes for preparing reagents include derivatizing polymeric materials to form acyl fluoride functionalities or derivatizing ethylene acrylic acid copolymers and ethylene methacrylic acid copolymers to form active acyl functionalities. Processes for immobilizing biopolymers include attaching natural or presynthesized biopolymers to activated solid support surfaces and directly attaching in a step-wise successive manner biomonomer units to a growing biopolymer chain attached to the solid support reagent.

Growing community of inventors

La Habra, CA, United States of America

Raymond C Milton