Cornell Research Foundation Inc.

Washington University

Other

Research, Incorporated

Myongji University Industry and Academia Cooperation Foundation

Ray J Wu

Saran A Narang

Ju-Kon Kim

Ajay K Garg

David McElroy

Tuan-Hua David Ho

Yang-Do Choi

Sang-Ik Song

Baek-Hie Nahm

In-Cheol Jang

Won-Bin Choi

Yeon-Seak Kim

Tuan-Hua D Ho

Chander P Bahl

Chung-Ho Kim

Christine W Wu

Deping Xu

The present invention relates to a method for increasing resistance of monocot plants against abiotic stress which comprises a step of transforming monocot plants with a recombinant plasmid containing a fused gene (TPSP) of trehalose-6-phosphate synthetase (TPS) gene and trehalose-6-phosphate phosphatase (TPP) gene to express the TPSP gene while maintaining normal growth and development characteristics. The present invention can increase the resistance of monocot plants against various stresses so that it can greatly contribute to the improvement of production and quality of valuable agricultural crops. The present invention also relates to a transgenic monocot plant, plant cell, or protoplast transformed with a nucleic acid encoding an enzyme for trehalose biosynthesis, under control of an inducible promoter, that increases tolerance to low temperature, salt, and water stress.

Method for increasing resistance of monocot plants against abiotic stresses, TPSP fusion enzyme gene constructs, and transformants

An isolated nucleic acid construct including a nucleic acid molecule encoding a light-labile, phytochrome A, a light-inducible promoter which is 5' to the nucleic acid molecule encoding a light-labile, phytochrome A, and a terminator region which is 3&#x2032; to the nucleic acid molecule encoding a light-labile, phytochrome A is disclosed. Methods for regulating a plant's canopy architecture and regulating a plant's seed yield, which involve transgenic plants or transgenic plant seeds including an isolated nucleic acid construct according to the present invention, are also disclosed.

Transgenic expression of a phytochrome a gene

The present invention relates to methods of constructing non-redundant, indexed, saturation, gene-disruption genomic libraries in plants and animals. The invention also relates to gene-disruption transformation plasmids for use in the method. The present invention also relates to plants, animals, and vertebrate and invertebrate cells transformed with such plasmids.

Plasmids and methods for construction of non-redundant, indexed, saturation, gene-disruption plant and animal libraries

The present invention relates to a method for conferring tolerance to salt stress and drought stress in a monocot plant including transforming the monocot plant with an expression cassette comprising at least one ABRC unit, a minimal promoter, and a DNA molecule that increases tolerance to salt stress and drought stress in plants, wherein the at least one ABRC unit, the minimal promoter, and a DNA molecule are operably linked together to permit expression of the DNA molecule. The present invention also relates to a transgenic monocot plant transformed with a DNA molecule that increases tolerance to salt stress and drought stress operably linked to at least one ABRC unit and a minimal promoter.

Method of making water stress or salt stress tolerant transgenic cereal plants

The current invention provides regulatory regions from the rice actin 2 gene. In particular, the current invention provides the rice actin 2 promoter and actin 2 intron. Compositions comprising these sequences are described, as well as transformation constructs derived therefrom. Further provided are methods for the expression of transgenes in plants comprising the use of these sequences. The methods of the invention include the direct creation of transgenic plants with the rice actin 2 intron and/or promoter directly by genetic transformation, as well as by plant breeding methods. The actin 2 sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Rice actin 2 promoter and intron and methods for use thereof

The present invention is directed to a method of producing a cereal plant cell or protoplast useful for regeneration of a water stress or salt stress tolerant cereal plant by transforming the cereal plant cell or protoplast with a nucleic acid encoding a late embryogenesis abundant protein. A transgenic cereal plant or cereal plant cell or protoplast transformed with a nucleic acid encoding a late embryogenesis abundant protein is also provided. An LEA protein gene, HVA1, from barley (Hordeum vulgare L.) was transformed into rice (Oryza sativa L.) plants. The resulting transgenic rice plants accumulate the HVA1 protein in both leaves and roots. Transgenic rice plants showed significantly increased tolerance to water stress (drought) and salt stress.

Production of water stress or salt stress tolerant transgenic cereal

The present invention describes the activity of a dicot wound inducible promoter in a monocot plant, the use of a monocot intron to enhance the activity of a dicot promoter in a transgenic monocot, and genetic constructs to introduce an exogenous genetic trait into a monocot plant using a wound inducible dicot promoter system.

Monocot having dicot wound-inducible promoter

The present invention describes the genomic nucleotide sequence of, and isolation of a strong intron-dependent promoter for, rice actin genes.

Rice actin gene and promoter

A human-like proinsulin gene and its analogs, have been synthesized by a combination of chemical and enzymatic methods. A number of different human-like proinsulin gene analogs with altered C-chains have also been designed and can be readily constructed as described. As a part of the strategy, an adaptor for trimming DNA has been designed, synthesized and used to recover the A-chain insulin gene with the desired sequence from a hybrid plasmid; a related adaptor for trimming DNA has been designed to shorten the C-chain gene or any gene. The synthetic proinsulin gene has been joined to a replicable cloning vehicle and the hybrid DNA transferred to a host cell. The transformed host cell has been shown to contain the desired human-like proinsulin gene.

Adaptors, and synthesis and cloning of proinsulin genes

Adaptor molecules have been prepared to comprise either start or stop signals for protein synthesis, in addition to recognition sites for restriction endonucleases. Separate adaptors may be used in a symmetrical duplex form. The start adaptor may include nucleotide base inserts to provide the correct reading frame of the triplet code in a DNA sequence with inappropriate reading frame. Insulin A-chain and B-chain genes of the human type, have been synthesized with the appropriate adaptor molecules provided on each end. The adapted DNA genes have been joined to replicable cloning vehicles and the hybrid DNA transferred to a host cell. The transformed host cell has been shown to contain the desired insulin gene.

Adaptor molecules for DNA and their application to synthesis of

Synthetic oligonucleotides have been designed and prepared which are useful in the molecular cloning of a variety of DNA molecules. By means of such oligonucleotides, genetic informational material, e.g., DNA, can be joined to a cloning vehicle and transferred into host cells by transformation. Additionally, a method for determining whether genetic informational material has been transferred into transformed host cells has been developed.

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Ithaca, NY, United States of America

Ray J Wu