Becton Dickinson and Company Limited

Ray A McMillian

Tobin J Hellyer

Thomas L Fort

Qimin You

Michael Porter

Hugh J Peck

Gregory A Richart

Chi Chen

Donald W Copertino

Panayiotis Zagouras

Courtney E Maus

Karen Eckert

Christing A Rostkowski

A region of theAP65-1 gene has been identified which is useful for performing amplification assays to determine specifically whetheris present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains ofand which does not show cross reactivity with the genomes of other microorganisms or with human DNA.

Assay for trichomonas vaginalis by amplification and detection of trichomonas vaginalis AP65-1 gene

A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.

Assay for detecting closely-related serotypes of human papillomavirus (HPV)

Assay forby amplification and detection ofAP65-1 gene

Assay forby amplification and detection ofgene

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A region of thecytotoxin gene has been identified which is useful for performing amplification assays to determine specifically whetheris present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains ofand which does not show cross reactivity with the genomes of other microorganisms or with human DNA.

The present invention relates to a method of ensuring the effectiveness of the extraction workup from a biological sample of nucleic acid. The inventive method is able to distinguish between possible defects in the extraction of nucleic acid from a sample and possible defects in a subsequent amplification step. The present invention also relates to a packaged array for extracting nucleic acid from a biological sample.

Use of an extraction control in a method of extracting nucleic acids

The present invention provides a method for preserving cells and nucleic acids in a sample by exposing the sample to a composition that causes an inhibitory affect on proteolytic agents and/or nucleic acid degradative agents in the sample. Such compositions that are useful in the method of the present invention include chelating agents such as sodium citrate, sodium borate, sodium fluoride and EDTA, that will bind trace metals necessary for proteolytic activity of proteolytic agents and/or nuclease activity of nuclease agents in a sample.

Method for preservation of cells and nucleic acid targets

Primers and probes derived from the 5&prime; untranslated region of the HCV genome which facilitate detection and/or quantification of all presently known genotypes of HCV. Disclosed sequences may be used in a variety of primer and probe constructs for amplification and/or detection of HCV nucleic acids.

Sequences and methods for detection of Hepatitis C virus

For use in nucleic acid amplification reactions, the detector oligonucleotides of the invention comprise a target binding sequence which is at least partially the same as the target binding sequence of an amplification primer present in the target amplification reaction, so that the detector oligonucleotide and the amplification primer compete for hybridization to the same sequence in the target. Hybridization of the amplification primer to the target upstream from the detector oligonucleotide generates a nickable restriction endonuclease recognition site. When this site is nicked and strand displacement occurs from the nick, both the,&prime; end of the amplification primer and the detector oligonucleotide are displaced. The displaced detector oligonucleotide may then be detected as an indication of the presence of the target sequence, for example by unfolding of a fluorescently labeled secondary structure present in the detector oligonucleotide to reduce fluorescence quenching.

Growing community of inventors

Timonium, MD, United States of America

Ray A McMillian