University of California

Hitachi Chemical Company, Ltd.

Hitachi Chemical Company America, Ltd.

Ravindra Lall Mehta

Masato Mitsuhashi

Taku Murakami

David M Ward

Satish P RamachandraRao

The present disclosure relates to methods of collecting exosomes and microvesicles (EMV) from urine and isolating corresponding mRNA in order to diagnose and treat acute kidney injury (AKI). In particular, certain embodiments relate to the method of capturing EMV from urine applied to a filter device that is capable of capturing EMV. Nucleic acids such as mRNA can be isolated from the EMV using an oligo(dT)-coated plate designed to accommodate the filter device and then used for further molecular analysis. Quantification of the collected nucleic acids may then be used in the diagnosis and/or treatment of IBD.

Method and device for diagnosing organ injury

The present invention provides a method of preventing blood from clotting in a filter during continuous arteriovenous or venovenous hemodialysis on a patient comprising administering to the blood an amount of citrate sufficient to prevent clotting in the filter. The invention preferably utilizes a dialysate having a sodium and calcium concentration below that of normal blood and an alkali concentration sufficiently low to reduce or prevent alkalosis.

Continuous hemodialysis using citrate

Growing community of inventors

La Jolla, CA, United States of America

Ravindra Lall Mehta