Indian Oil Corporation Limited

Department of Biotechnology

Department of Biotechnology Ministry of Science & Technology

Ravi Prakash Gupta

Sankara Sri Venkata Ramakumar

Suresh Kumar Puri

Anshu Shankar Mathur

Deepak Kumar Tuli

Ravindra Kumar

Alok Satlewal

Simranjeet Kaur Sandhu

Mukund Adsul

Reeta Rani Singhania

Prakash Chandra Sahoo

Ruchi Agrawal

Manoj Kumar

Preeti Mehta

Deepak Saxena

Ajay Kumar Sharma

Ajit Singh

Manas Ranjan Swain

Tirath Raj

Rekha Rani

Manoj N Kumar

Biswajit Basu

Srikanth Sandipam

Surbhi Semwal

Periyasamy Sivagurunathan

Ankita Kandpal

Anshu Shanker Mathur

Dilip Singh

Anil Kumar Patel

Manali Kapoor Kakkar

Ruchi Gaur

Jitendra Kumar Saini

The present disclosure relates to a process for producing levulinic acid from the technical lignin. The technical lignin is rich in lignin and ash and do not have any other use and usually incinerated as a waste management strategy. During the incineration process, the cellulose also gets burnt and wasted. In the present disclosure, there is provided an economical and environmentally friendly process to produce levulinic acid from the technical lignin by using a synergistic combination of catalysts for high yields and high titer with acid recycling in a cost-effective manner.

Method for producing levulinic acid

The present invention relates to a novel phase transfer solvent composition for enhanced COand/or HS capture from flue gas and biogas having various gaseous compositions. Further, the present invention provides a process of preparing the phase transfer solvent composition of the present invention.

Enzymatic phase transfer solvent for CO/HS capture

The present invention relates to a process for microbial synthesis process having less water consumption and yielding desired product profile. More particularly, the present invention relates to an improved process of microbial synthesis using ultra fine nutrient mist in a specially designed biofilm-bioreactor under controlled conditions. The present invention also relates to an apparatus for microbial synthesis and preparation of optimized biofilm for continuous product formation.

Process for microbial synthesis and an apparatus thereof

The present invention relates to a process for simultaneous production of citric acid and cellulolytic enzymes. The batch process comprising (i) adding slurry of a pre-treated lignocellulosic biomass or cellulose in a fermentation media; (ii) inoculating 10% (v/v) active liquid seed culture ofMRJ-16 in the fermentation media of step (i); (iii) subjecting the culture of step (ii) to fermentation in an aerated fermenter at a temperature of 28-33° C. for a duration of 96 hours; and (iv) collecting enzyme broth after fermentation of step (iii) to obtain citric acid and cellulolytic enzymes. The batch and fed-batch process of the present invention results in high titer production of citric acid and cellulolytic enzymes in a single step and using single microbial strain.

Process for simultaneous production of citric acid and cellulolytic enzymes

The present invention relates to a process for producing enriched algal biomass having high lipid productivity. More particularly, the present invention provides a process for obtaining an enriched biomass with omega-3 fatty acids by using a microalgal strainMTCC 5890 in a unique media composition and substrate residual band in a steady state continuous fermentation. The process of the present invention results in high biomass and lipid productivity.

Process for production of enriched algal biomass

The present invention provides an improved process for high titer lactic acid production using simultaneous saccharification and co-fermentation process using acid treated lignocellulosic biomass. The process comprises of pretreating lignocellulosic biomass comprising C5 and C6 sugars with dilute acid and superheated steam at a temperature ranging from 150-210° C.; neutralizing the pretreated lignocellulosic biomass of step (i) with NaOH pellets; and adding cellulase enzyme in the range of 1.5 to 10 FPU/g, bacteria, nutrients and buffering agent to the neutralized slurry obtained in step (ii) for hydrolysis and co-fermentation of C5 and C6 sugars at a temperature ranging from 40-45° C. for a period of 120-144 hours to obtain lactic acid. The process of the present invention requires simple nutrients and results in high titer lactic acid production within shortest duration of time.

Process for second generation lactic acid production

The present invention provides a method for the simultaneous enhancement in biomass and lipids containing omega-3-fatty acids ofmicroalgae in a single step using synergistic effect of chemical mixture in appropriate proportion in production medium. The process discloses enriching the biomass of microalgae with high value lipids by subjecting the microalgal cells in growth medium supplemented with unique combination of chemical modulators and carbon substrates in the presence of nitrogen. The present invention also provides a novel strainsp. (MTCC 5980) for use in continuous aerobic fermentative lipid production process for enhancing high value lipids like Docosahexaenoic acid (DHA), Docasapentaenoic acid (DPA), Eicosapentaenic acid (EPA) and lipids for biodiesel.

Methods and formulations for enhancing high value lipids

The present invention relates to a biocompatible task specific hybrid solvent (TSHS) composition that synergistically increases the carbon capture efficiency and utilizes less energy for COdesorption over the existing solutions. Further, the process of synthesizing such composition is discussed in this disclosure. Also, the present disclosure provides a process that involves a task specific hybrid solvent (TSHS) composition for selective capturing COfrom any gaseous stream.

Solvent composition for COcapture and a process mediated thereof

The present invention relates to a booster composition for enhancing the biogas yield and stabilizing the operation of a biomethanation plant, a method for the preparation of said booster composition and a method for enhancing the biogas yield and stabilizing the operation of a biomethanation plant using said composition by ameliorating the effect of shock conditions like pH and temperature. The booster composition comprises of specifically defined constituents selected from controlled release agents, redox potential balancers, direct interspecies electron transfer (DIET) improvers, micronutrient balancers, and Co-factors.

Booster composition to improve biogas yield and to stabilize the digester performance

An integrated approach for utilizing waste products of 2G bio-refineries to fractionate the lignin of high purity. The present invention also provides a method of recycling of two waste products (2-G ethanol residue as the substrate (LRBR) and fusel oil/synthetic fusel oil (SFO) as one of the solvent) of the biorefineries in a beneficial manner to fractionate the lignin of high purity. The present method of separating high purity lignin comprises fractioning extractive-free lignin rich residue with a solvent, wherein the solvent is a mixture of SFO/Fusel oil and Formic acid. An optimized ratio of waste fusel oil and formic acid gave more than &#x3e;85% yields of high purity lignin.

Method to valorize 2G bioethanol waste streams

The present invention discloses a process of xylitol production from pure xylose, xylose containing liquid extract from the acid pretreated biomass (LEPB) and co-production of ethanol and xylitol from dilute acid pretreated lignocellulosic biomass by naturalDBT-IOC S24 (MTCC25086). The present invention also discloses a process of xylitol production wherein a thermo and inhibitor tolerantDBT-IOC S24 (MTCC25086) natural strain has been induced to produce xylitol from xylose at defined process parameters using xylose containing diluted acid pretreated biomass as well as from xylose containing synthetic media in presence of glucose, lignocelluloses biomass derived xylitol inducer maintaining aeration at 1VVM and pH at 5.0 in the medium.

Process for xylitol production from xylose

The present invention relates to a mutant fungal strain of'MRJ-16' characterized by the ability to produce high titer of enzyme mixture comprising FPase, CMCase, Cellobiase, &#x3b2;-glucosidase, endoglucanase, &#x3b1;-L arabinofuranosidase, &#x3b2;-xylosidase, xylanase, pectinase, cellobiohydrase and oxidases and produce enzymes in the presence of a catabolite repressor molecule like glucose and/or xylose. The titer of enzyme mixture produced using mutant fungal strain MRJ-16 is at least two fold higher than naivestrain NCIM 1228, when used in a fermentation process. The mutant strain 'MRJ-16' with high hydrolytic activity and catabolite derepressed character is having application in the method of degrading or saccharifying biomass to produce valuable products for example-bioethanol.

Method for preparation and screening of fungal mutant with high hydrolytic activity and catabolite derepressed character

A method for preparing a hyper-cellulolytic catabolite derepressed mutants of ascomycetes fungus, especially variants of. Selection media used to isolate such variants include amorphous cellulose and a high concentration of glucose. Cellulase activities of mutant ID-10, in particular such as FPase and &#x3b2;-glucosidase were 1.5 times higher thanMRJ-16 (parent). Furthermore, fungal mutant morphology was changed and no pH adjustment was required throughout the enzyme production process.

Method for preparation of fungal mutant with high hydrolytic activity

The present invention relates to a method for obtaining a high titer of enzyme mixture comprising cellulases, hemicellulases and &#x3b2;-glucosidases in reutilization of waste water generated during hot water extraction of lignocellulosic biomass or biorefinery waste water usingMRJ-16 mutant strain. The cellulose or lignocellulosic biomass used in the fermentation process is selected from the group consisting of rice straw, wheat straw, corn stover, cotton stalk or a combination thereof. The enzyme mixture obtained by the present process is used for the saccharification of acid pretreated lignocellulosic biomass.

Bio-refinery waste utilization for enzyme production using novel penicillium funiculosum MRJ-16 fungal strain

An advantageous process for pre-treating lignocellulosic biomass to hydrolysable polysaccharide enriched biomass with reduced amount of inhibitors of catalysis comprising steps of: providing a feedstock comprising cellulosic biomass; chopping of cellulosic biomass to have a cellulosic biomass feed material of uniform size; feeding of uniform sized feed material to a horizontal counter current extraction unit; removing excess of water from the feed material obtained from the extraction unit; soaking the extruded feed material obtained from the extraction unit; optionally removing excess of water from the feed material obtained after acid soaking; steam pre-treating the feed material obtained after removing excess of water from the acid soaked feed material; neutralizing the slurry obtained from the steam pre-treating; and optionally saccharifying the neutralized slurry.

Growing community of inventors

Faridabad, India

Ravi Prakash Gupta