Pacific Biosciences of California, Inc.

Ravi Dalal

Stephen Wilson Turner

Jonas Korlach

Paul M Lundquist

Robin A Emig

John Lyle

John S Eid

Fred C Christians

Adrian Nielsen Fehr

Benjamin A Flusberg

Jeremy Gray

Lei Sun

Stephen P Dudek

Khai Luong

Steven Lin

Robert Sebra

Methods for non-random loading of single analyte molecules into array structures are provided. The methods allow for distribution of a population of target molecules into a plurality of size confined regions such as wells. Sizing moieties are linked to individual target molecules. The sizing moieties are of sufficient size, relative to the size-confined reaction or observation regions, such that only a selected number of sizing moieties will fit into the size confined regions. The confined regions and the sizing moieties or target molecules comprise a selected charge that allow for controlling the loading of the sizing moities.

Single molecule loading methods and compositions

Methods, systems and their components for monitoring fluorescent signals and particularly transient fluorescent signals from reaction mixtures of interest, which methods and systems employ modulated excitation light sources to reduce impacts of excessive illumination on the reaction components or the data obtained therefrom.

Methods and systems for analysis of fluorescent reactions with modulated excitation

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Menlo Park, CA, United States of America

Ravi Dalal