Lanzatech New Zealand Limited

Lanzatech, Inc.

Rasmus Overgaard Jensen

Michael Koepke

James Bruce Yarnton Haycock Behrendorff

Ryan Edward Hill

Alexander Paul Mueller

Ching Leang

Zachary Robert Cowden

Darmawi Juminaga

James Daniell

Provided herein are microorganisms and methods for fermentative production of β-ketoadipate from gaseous substrates such as carbon dioxide (CO), carbon monoxide (CO), and/or hydrogen (H). Additionally, the processes provided herein are methods for producing polymers containing β-ketoadipate, that can potentially enable a circular economy by diverting waste, e.g., plastic waste.

Fermentative production of B-ketoadipate from gaseous substrates

The disclosure provides genetically engineered microorganisms and methods for improved biological production of ethylene glycol and precursors of ethylene glycol. The microorganism of the disclosure produces ethylene glycol or a precursor of ethylene glycol through one or more of 5,10-methylenetetrahydrofolate, oxaloacetate, citrate, malate, and glycine. The disclosure further provides compositions comprising ethylene glycol or polymers of ethylene glycol such as polyethylene terephthalate.

Microorganisms and methods for improved biological production of ethylene glycol

The invention provides genetically engineered microorganisms and methods for the biological production of ethylene glycol and precursors of ethylene glycol. In particular, the microorganism of the invention produces ethylene glycol or a precursor of ethylene glycol through one or more of 5,10-methylenetetrahydrofolate, oxaloacetate, citrate, malate, and glycine. The invention further provides compositions comprising ethylene glycol or polymers of ethylene glycol such as polyethylene terephthalate.

Microorganisms and methods for the biological production of ethylene glycol

The invention relates to a genetically engineered bacterium having an enzyme that converts 3-hydroxyisovaleryl-CoA to 3-hydroxyisovalerate and an enzyme that converts 3-hydroxyisovalerate to isobutylene. Typically, the bacterium is capable of producing isobutylene from a gaseous substrate containing CO, CO, and/or H, such as syngas or an industrial waste gas.

Genetically engineered bacterium for the production of isobutylene

The invention relates to a genetically engineered bacterium having an enzyme that converts acetyl-CoA to acetoacetyl-CoA, an enzyme that converts acetoacetyl-CoA to 3-hydroxybutyryl-CoA, and an enzyme that converts 3-hydroxybutyryl-CoA to 3-hydroxybutyrate. The bacterium may also have enzymes to produce other downstream products, such as 3-hydroxybutyryaldehyde, and 1,3-butanediol. Typically, the bacterium is capable of producing these products from a gaseous substrate, such as syngas or an industrial waste gas.

Genetically engineered bacterium for the production of 3-hydroxybutyrate

The invention relates to a genetically engineered bacterium comprising an energy-generating fermentation pathway and methods related thereto. In particular, the invention provides a bacterium comprising a phosphate butyryltransferase (Ptb) and a butyrate kinase (Buk) (Ptb-Buk) that act on non-native substrates to produce a wide variety of products and intermediates. In certain embodiments, the invention relates to the introduction of Ptb-Buk into a C1-fixing microoorgansim capable of producing products from a gaseous substrate.

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Skokie, IL, United States of America

Rasmus Overgaard Jensen