Council of Scientific and Industrial Research

Rashmita Sahoo

Paramvir Singh Ahuja

Sanjay Rai Kumar

The invention relates to a novel purified isozyme of an autoclavable superoxide dismutase extracted from the plantLodd. Var.said isozyme having the following characteristics, Oscavenging activity remains same before and after autoclaving; scavenges Ofrom sub-zero temperature of &#x2212;20&#xb0; C. to high temperature of +80&#xb0; C.; Oscavenging activity at 25&#xb0; C. for 30 days without adding any stabilizing agent such as polyols or sugars; Oscavenging activity in the presence of saline (0.9% sodium chloride) to 61.8% of the control (without 0.9% sodium chloride), stable at 4&#xb0; C. for at least 8 months; contamination free and infection free from any living micro- and/or macro-organism after autoclaving; possesses temperature optima at 0&#xb0; C.; possesses a molecular weight of 33 kD under non-denaturating conditions; possesses a molecular weight of 36 kD under denaturating conditions; has clear peaks in UV range at 268 and 275 nm; has an enzyme turnover number of 19.53&#xd7;10% per nmol per min at 0&#xb0; C.; and requires Cu/Zn as a co-factor, method for the preparation of the purified isozyme of autoclavable superoxide dismutase and formulations containing the said autoclavable superoxide dismutase.

Isozyme of autoclavable superoxide dismutase (SOD), a process for the identification and extraction of the SOD and use of the said SOD in cosmetic, food, and pharmaceutical compositions

The invention relates to a novel purified isozyme of an autoclavable superoxide dismutase extracted from the plant Var. said isozyme having the following characteristics, O scavenging activity remains same before and after autoclaving; scavenges O from sub-zero temperature of &minus;20&deg; C. to high temperature of &plus;80&deg; C.; O scavenging activity at 25&deg; C. for 30 days without adding any stabilizing agent such as polyols or sugars; O scavenging activity in the presence of saline (0.9% sodium chloride) to 61.8% of the control (without 0.9% sodium chloride), stable at 4&deg; C. for at least 8 months; contamination free and infection free from any living micro- and/or macro-organism after autoclaving; possesses temperature optima at 0&deg; C.; possesses a molecular weight of 33 kD under non-denaturating conditions; possesses a molecular weight of 36 kD under denaturating conditions; has clear peaks in UV range at 268 and 275 nm; has an enzyme turnover number of 19.53&times;10 % per nmol per min at 0&deg; C.; and requires Cu/Zn as a co-factor, method for the preparation of the purified isozyme of autoclavable superoxide dismutase and formulations containing the said autoclavable superoxide dismutase.

Isozyme of autoclavable superoxide dismutase (SOD), a process for the identification and extraction of the SOD in cosmetic, food and pharmaceutical compositions

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Himachal Pradesh, India

Rashmita Sahoo