Biogen Ma Inc.

Biogen Idec Ma Inc.

Gloriana Therapeutics, Inc.

Rashmi Rohit Kshirsagar

Alan Benjamin Gilbert

R Blake Pepinsky

Dingyi Wen

David Evans

Karin Lucas

Kyle McElearney

Terrence Michael Dobrowsky

The present disclosure pertains to a mammalian cell culture genetically modified to express, and which expresses, a neublastin antibody polypeptide, or fragment thereof, in the culture, and to a neublastin antibody polypeptide, or fragment thereof, made by a mammalian cell culture genetically modified to express, and which expresses, the neublastin antibody polypeptide, or fragment thereof.

Mammalian cell culture-produced neublastin antibodies

The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.

Methods of preventing and removing trisulfide bonds

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Ashland, MA, United States of America

Rashmi Rohit Kshirsagar