Illumina Cambridge Limited

Raquel Maria Sanches-Kuiper

Geoffrey Paul Smith

David James Earnshaw

David Mark Dunstan Bailey

Harold Philip Swerdlow

Roberto Rigatti

Shankar Balasubramanian

Tobias William Barr Ost

Hardold Swerdlow

The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogues bearing substituents at the 3' position of the sugar moiety that are larger in size than the naturally occurring 3&#x2032; hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.

Modified polymerases for improved incorporation of nucleotide analogues

Modified DNA polymerases have an affinity for DNA such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate DNA templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-DNA complexes in each reaction cycle. The modified polymerases may be used in a number of DNA sequencing applications, especially in the context of clustered arrays.

Polymerases

The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogs bearing substituents at the 3' position of the sugar moiety that are larger in size than the naturally occurring 3&#x2032; hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.

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Nr Saffron Walden, United Kingdom

Raquel Maria Sanches-Kuiper