Duke University

Raphael Valdivia

Dewey G McCafferty

Ine Jorgensen

Lauren Davey

Sena Bae

Maria M Bednar

Per Malkus

The present disclosure provides methods and systems for genetically altering and screeningbacteria, including. The methods also provide genetically altered bacteria, libraries of genetically altered bacteria and use of such bacteria for treatment of diseases.

Systems and methods for genetic manipulation ofspecies

Embodiments of the present disclosure relate generally to the genetic analysis of intractable microbes. More particularly, the present disclosure provides materials and methods that incorporate chemical mutagenesis, phenotypic selection, suppression analysis, and genomic sequencing-based mutational mapping, to identify novel genetic regulators in previously intractable organisms, such as microbes that constitute the human microbiome. Given the paucity of experimental tools to manipulate bacterial genomes, there is a need for improved methods for determining how microbial communities assemble and influence human and environmental health.

Systems and methods for genetic analysis of intractable microbes

An antichlamydial agent comprising an inhibitor of Chlamydial Protease-like Activity Factor (CPAF). The inhibitor of CPAF can comprise a CPAF inhibitory segment and can optionally include one or more additional residues or domains. Also provided are compositions comprising an inhibitor of CPAF, methods of identifying an inhibitor of CPAF, and methods of treating ainfection in a subject comprising administering an inhibitor of CPAF or a composition comprising an inhibitor of CPAF to the subject.

Growing community of inventors

Carrboro, NC, United States of America

Raphael Valdivia