Association for the Advancement of Tissue Engineering and Cell Based Technologies and Therapies-a4tec

Raphael Faustino Canadas

Rui Luis Gonçalves Dos Reis

Joaquim Miguel Antunes De Oliveira

Alexandra Margarida Pinto Marques

A rotational dual chamber bioreactor for cell culture in bi- and multi-layered scaffolds, in the context of tissue engineering (TE) and regenerative medicine strategies is disclosed, which comprises at least two dual culture chambers, a multiposition magnetic stirrer plate, and at least two flow pumps coupled to a monitor device to register biochemical parameters such as pH, pO, glucose and urea sensors and physical parameters like pressure. A central barrier, with a hole to insert the bilayer scaffold, is used to culture cells with different conditions in each compartment of the chamber, avoiding medium exchange. Each chamber can rotate 180&#xb0; (horizontal), which is promoted by magnetic stirrers, the multiposition stirrer plate is adapted dimensionally to the culture plate dimensions and also allows 180&#xb0; vertical rotation. Each dual chamber has detachable caps for the top and the bottom chambers, the top cap to compress the scaffold thereby providing compressive stimulus with torsion simultaneously.

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Guimaraes, Portugal

Raphael Faustino Canadas