University of Michigan

Other

The State University of New York

Health Research, Inc.

Raoul Kopelman

Heather Clark

Brandon H McNaughton

Susan Barker

Ravindra Kumar Pandey

Aaron J Lewis

Anurag Gupta

Munawwar Sajjad

Gwangseong Kim

Martin A Philbert

Zhong-You Joe Shi

Roy Clarke

Stephen J Parus

Eric Monson

Bjorn Thorsrud

Weihong Tan

Yong-Eun Koo Lee

Jerome Kalifa

Hyung Ki Yoon

Uma Mahesh R Avula

Remy Elbez

Mark A Burns

Vadim N Savvateev

Russell Witte

Joseph Shinar

Alan Hunt

Paivo Kinnunen

Yunzi Li

Shai Ashkenazi

Jon H Friedl

Ramon Torres-Isea

Zoe Chen-Esterlit

Jonathan W Aylott

Irene Sinn

Theodore Albertson

Jeffrey Anker

Caleb Behrend

Katherine M Tyner

Thomas D Horvath

Ariel Hecht

The present invention relates to nanoparticles. In particular, the present invention provides nanoparticles for clinical (e.g., targeted therapeutic), diagnostic (e.g., imaging), and research applications in the field of cardiology. For example, in some embodiments, the present invention provides a method of treating (e.g., ablating) cardiac tissue, comprising: a) contacting an animal with a nanoparticle comprising a matrix, a toxic (e.g., ablative) agent (e.g., sonosensitizer, chemotherapeutic agent (e.g., doxorubicin or cisplatin), or photosensitizer), and a cardiac targeting moiety; and b) administering an activator of the toxic agent (e.g., light, chemical (e.g., pharmaceutical agent) or ultrasound) to at least a portion of the cardiac tissue (e.g., heart) of the animal to activate the toxic agent.

Systems and methods for targeted imaging and ablation of cardiac cells

A composition comprising PAA nanoparticles containing a post loaded tetrapyrollic photosensitizer and a postloaded imaging agent and methods for making and using same.

Multifunctional nanoplatforms for fluorescence imaging and photodynamic therapy developed by post-loading photosensitizer and fluorophore to polyacrylamide nanoparticles

The disclosure provides a label-free viscosity-based analyte detection system using paramagnetic beads as an asynchronous magnetic bead rotation (AMBR) microviscometer. It is disclosed herein that the bead rotation period is linearly proportional to the viscosity of a solution comprising analytes surrounding the paramagnetic bead. Optical measurement of asynchronous microbead motion determines solution viscosity precisely in microscale volumes, thus allowing an estimate of analyte concentration. The results demonstrate the feasibility of viscosity-based analyte detection using AMBR in microscale aqueous volumes.

Asynchronous magnetic bead rotation (AMBR) microviscometer for analysis of analytes

Identification, quantification and characterization of biological micro- and nano-systems is enabled by magnetically spinning these natural, non-magnetic systems with the aid of induced magnetization. Biofriendly magnetic micro- and nano-labels enable magnetorotation in extremely weak electromagnetic fields. The spinning of these micromotors can be observed by a simple, CD-like, optical tracking system. The spinning frequency response enables real-time monitoring of single (cancer) cell morphology, with sub-microscopic resolution, yielding previously undeterminable information. Likewise, it enables super-low detection limits for any (cancer) biomarker.

Magnetically induced microspinning for super-detection and super-characterization of biomarkers and live cells

The present invention relates to biological sensors. In particular, the present invention relates to the use of remotely driven nonlinear rotation of particles (e.g., magnetic particles) for detection of cells such as microorganisms (e.g., bacteria and viruses). The present invention further relates to the use of remotely driven nonlinear rotation of particles for measurement of physical properties of a solution (e.g., viscosity).

Non-linear rotation rates of remotely driven particles and uses thereof

A PAA nanoparticle containing a covalently linked fluorescent dye and a post-loaded tetrapyrrolic photosensitizer.

PAA nanoplatforms containing fluorophores and targeted moieties covalently linked and photosensitizer post-loaded

Described herein are various methods, devices and systems for performing asynchronous magnetic bead rotation (AMBR) to detect and monitor cellular growth and/or behavior. Cluster rotation of magnetic particles for AMBR is descried. In particular, described herein are systems for the parallel analysis of multiple wells of a sample plate. Also described herein are methods for controlling the illumination and imaging of rotating magnetic particles.

Asynchronous magnetic bead rotation sensing systems and methods

The present invention relates to modulated (e.g., magnetically modulated) chemical sensors. In particular, the present invention relates to particles comprising fluorescent indicator dyes and methods of using such particles. Magnetic fields and/or Brownian motion modulate an optical property of the particle to distinguish it from background signals. The present invention thus provides improved methods of detecting a wide variety of analytes in fluids, fluid samples, cells and tissues.

Modulated physical and chemical sensors

A composition comprising PAA nanoparticles containing a post loaded tetrapyrollic photosensitizer and an imaging agent and methods for making and using same.

PAA nanoparticles for enhancement of tumor imaging

A universal, wireless, nano-optical voltmeter comprises an organic core having at least one voltage-sensitive dye and at least one polymeric shell substantially surrounding the organic core. The nano-optical voltmeter can detect electric fields in cells. The nano-optical voltmeter allows three-dimensional E field profiling throughout the entire volume of living cells. The nano-optical voltmeter may be calibrated externally and then applied for E field determinations inside any live cell or cellular compartment, with no further calibration steps.

Universal, wireless, nano-optical voltmeters

A method of measuring fluorophore excited state lifetimes comprising initiating an excitation laser pulse at a dye to excite dye molecules of the dye from a ground state to an excited state and initiating a probing pulse at the dye molecules thereby generating a first set of photoacoustic waves at a first time delay resulting in a first intensity point. The process can be repeated, optionally introducing a second excitation laser pulse, to generate a second set of photoacoustic waves at a second time delay resulting in a second intensity point. The data can be analyzed to determine a slope between the first intensity point and the second intensity point.

Photoacoustic indicators

Optical fiber sensors and fiberless optical sensors for measuring analytes, and in particular nitric oxide, are described utilizing metals, and more particularly, metal colloids. Proteins (or fragments thereof) with selective binding are immobilized on metal particles. The proteins may be dye-labeled for increased sensitivity.

Optical sensors for the detection of nitric oxide

Optical fiber sensors and fiberless optical sensors for measuring analytes, and in particular nitric oxide, are described utilizing metals, and more particularly, metal colloids. Proteins (or fragments thereof) with selective binding are immobilized on metal particles. The proteins may be dye-labeled for increased sensitivity. Additionally, metals functionally linked to reporter dyes are described in addition to the incorporation of reference compounds for ratiometric measurements.

Fiberless optical sensors (plasticized PVC, acrylamide or gold particles) are described having a size ranging from between approximately 1 micrometer and 1 nanometer in diameter. The sensors comprise ionophores useful for the detection of intracellular analytes.

Optical fiberless sensors

Growing community of inventors

Ann Arbor, MI, United States of America

Raoul Kopelman