Shandong University

Bloomage Biotechnology Corporation Limited

Ranran Du

Xueping Guo

Fengshan Wang

Jianqun Deng

Zhen Lu

Yuanjun Sun

Juzheng Sheng

Liu Sun

A heparin skeleton synthase originates from, with an amino acid sequence as shown in SEQ ID NO.2 and a nucleotide sequence of the coding gene as shown in SEQ ID NO.1. Its recombinant expression level is 6.8 times that of the existing heparin skeleton synthase KfiA fromK5, and total enzyme activity per fermentation liquor is 5.22 times that of the heparin skeleton synthase KfiA. The heparin skeleton synthase mutants obtained through site-directed mutagenesis of the sites No. 16, No. 25, No. 30, No. 111, No. 165, and No. 172 in the amino acid sequence of the said heparin skeleton synthase all have high expression levels.

Heparin skeleton synthase and its mutants and application

Growing community of inventors

Jinan, China

Ranran Du