Butamax Advanced Biofuels LLC

E.i. Dupont De Nemours and Company

Butamax (Tm) Advanced Biofuels LLC

Ranjan Patnaik

Lori Ann Maggio-Hall

Michael Charles Grady

Arthur Leo Kruckeberg

Hongxian He

Yougen Li

Mark J Nelson

Steven Cary Rothman

Lixuan Lisa Huang

Daniel P Okeefe

Jessica McElvain

Ritu Bhalla

Vasantha Nagarajan

Wonchul Suh

Dennis Flint

Daniel P O'Keefe

Larry Cameron Anthony

Fateme Sima Sariaslani

Steven D Doig

Mehmedalija Jahic

Kakasaheb Suresh Konde

Andrew C Eliot

Arie Ben-Bassat

David J Lowe

Gail K Donaldson

Tina K Van Dyk

Anthony Arthur Gatenby

Lisa L Huang

Bhaskar Bhadra

Katharine J Gibson

Anthony A Gatenby

Provided herein are processes for producing an improved culture of cells comprising an engineered butanol biosynthetic pathway. The processes comprise (a) providing a cell culture of recombinant microorganisms comprising an engineered butanol biosynthetic pathway, wherein the engineered butanol biosynthetic pathway is minimal or not activated; and (b) growing the culture of recombinant microorganisms under adaptive conditions whereby pathway activation is increased to produce an improved cell culture and whereby the improved cell culture is capable of continuing to grow in fermentation.

Partial adaption for butanol production

The invention relates to the fields of industrial microbiology and alcohol production. The invention also relates to the development of a microorganism capable of producing fermentation products via an engineered pathway, and uses of the microorganism. The invention also relates to the methods to improve cell viability and productivity and the use of recycling and acid washing to increase the yield of fermentation products.

Fermentative production of alcohols

Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.

Host cells and methods for production of isobutanol

Partial adaptation for butanol production

Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.

Method for the production of isobutanol by recombinant yeast

A method for producing butanol through microbial fermentation, in which the butanol product is removed during the fermentation by extraction into a water-immiscible organic extractant in the presence of at least one electrolyte at a concentration at least sufficient to increase the butanol partition coefficient relative to that in the presence of the salt concentration of the basal fermentation medium, is provided. The electrolyte may comprise a salt which dissociates in the fermentation medium, or in the aqueous phase of a biphasic fermentation medium, to form free ions. Also provided is a method and composition for recovering butanol from a fermentation medium.

Method for producing butanol using extractive fermentation with electrolyte addition

A method of making butanol from at least one fermentable carbon source that overcomes the issues of toxicity resulting in an increase in the effective titer, the effective rate, and the effective yield of butanol production by fermentation utilizing a recombinant microbial host wherein the butanol is extracted into specific organic extractants during fermentation.

Method for producing butanol using two-phase extractive fermentation

Methods for the microbial production of para-hydroxycinnamic acid (pHCA) and cinnamic acid (CA) are provided. Microbes producing either tyrosine or phenylalanine are grown in the presence of either tyrosine ammonium lyase or phenylalanine ammonium lyase respectively where some part of the fermentation is accomplished at alkaline pH. The process results in greater yields and higher rates of para-hydroxycinnamic acid (pHCA) and cinnamic acid (CA) production as compared with fermentation exclusively at physiological pH.

Methods for the preparation of para-hydroxycinnamic acid and cinnamic acid at alkaline pH

An enteric bacterial strain was engineered to over-produce L-tyrosine using a one-step method. The pheA-tyrA chromosomal region of the bacterial genome was replaced with an engineered chromosomal segment, resulting in inactivation of the pheA coding region and strong expression of the tyrA coding region, resulting in high levels of L-tyrosine production.

Method for producing an L-tyrosine over-producing bacterial strain

Growing community of inventors

Newark, DE, United States of America

Ranjan Patnaik