Wyeth

Janssen Alzheimer Immunotherapy

Pfizer Corporation

Ranganathan Godavarti

Timothy Iskra

Paul Brown

Brian D Kelley

James Edward Booth

Shujun Sun

Suresh Vunnum

Mary Beth Switzer

Tianning Yu

Jonathan Coffman

Michael Shamashkin

Jonathan Lee Coffman

Jon Coffman

Tim Iskra

The present disclosure provides methods for purifying products from a fluid. In some embodiments, provided purification methods use a combination of purification modes (e.g., protein A and ion exchange) operated in tandem, wherein at least one of the modes utilizes weak partitioning. In some embodiments, provided purification methods operate under robust conditions in which a degree of binding between a product and resin is maintained despite variations in operating parameters.

Tandem purification of proteins

This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid containing one or more impurities. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.

Method of weak partitioning chromatography

The present application provides methods of purifying A&#x3b2; binding proteins having a Fc region, for example, anti-A&#x3b2; antibodies or antibody fusions, by adsorbing the A&#x3b2; binding protein to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed A&#x3b2; binding protein. The present application also features methods of eluting the purified A&#x3b2; binding protein as well as the incorporation of the methods within a purification train. Kits comprising components for carrying out the methods and instructions for use are also provided.

Methods of purifying anti A &#x3b2; antibodies

The present application provides methods of purifying polypeptides having a Fc region, for example, antibodies or antibody fusions, by adsorbing the polypeptides to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed polypeptides. The present application also features methods of eluting the purified polypeptide as well as the incorporation of the methods within a purification train. Kits comprising components for carrying out the methods and instructions for use are also provided.

Methods of purifying Fc region containing proteins

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Burlington, MA, United States of America

Ranganathan Godavarti