Isis Pharmaceuticals, Inc.

Ranga Sampath

Richard Holmes Griffey

David J Ecker

John McNeil

Eric E Swayze

Steven A Hofstadler

Stanley T Crooke

Venkatraman Mohan

Methods for the identification of compounds which modulate, either inhibit or stimulate, biomolecules are provided. Nucleic acids, especially RNAs are preferred substrates for such modulation. The present methods are particularly powerful in that they provide novel combinations of techniques which give rise to compounds, usually 'small' organic compounds, which are highly potent modulators of RNA and other biomolecular activity. In accordance with preferred aspects of the invention, very large numbers of compounds may be tested essentially simultaneously to determine whether they are likely to interact with a molecular interaction site and modulate the activity of the biomolecule. Pharmaceuticals, veterinary drugs, agricultural chemicals, industrial chemicals, research chemicals and many other beneficial compounds may be identified in accordance with embodiments of this invention.

Molecular interaction sites of interleukin-2 RNA and methods of modulating the same

Methods of identifying molecular interaction sites in eukaryotic and prokaryotic nucleic acids, especially RNA, are described. Secondary structural elements are identified from highly conserved sequences. Methods of preparing databases relating to such molecular interaction sites are also provided herein as are databases themselves. Therapeutic, agricultural, industrial, and other applicability results from interaction of such molecular interaction sites with &ldquo;small&rdquo; and other molecules.

Identification of molecular interaction sites in RNA for novel drug discovery

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San Diego, CA, United States of America

Ranga Sampath