Biofire Diagnostics, LLC

University of Utah Research Foundation

Idaho Technology, Inc.

Roche Diagnostics Gmbh

Biofire Diganostics, LLC

F. Hoffmann-la Roche Ag

Kirk M. Ririe

Carl T. Wittwer

Randy P. Rasmussen

Randy P Rasmussen

Kirk M Ririe

Mark Aaron Poritz

Carl Thomas Wittwer

Gary Clark Kessler

Michael R Newswander

Stewart Benjamin Smith

David J Eyre

David E Jones

Thomas Charles Robbins

Robert John Crisp

Andrew Clinton Hemmert

Elizabeth Barker Campbell

Deepika Marine deSilva

David R Hillyard

Brian E Caplin

Karim Tabiti

Richie Soong

Wade R Stevenson

Marla Lay

Gisela Betzl

John G Ward

Hallegh Page Millward

Haliegh Page Millward

Deepika Marine Desilva

Elizabeth Diana Barker Campbell

Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include nucleic acid amplification and detection and immuno-PCR.

Self-contained biological analysis

Methods and devices are provided for simultaneously amplifying a plurality of sample wells for a predetermined amount of amplification, detecting whether amplification has occurred in a first set of the wells, amplifying for an additional amount of amplification and detecting whether amplification has occurred in a second set of the wells. Methods are also provided for analyzing a target nucleic acid sequence using melt curves that were generated in a plurality of amplification cycles.

Multiple amplification cycle detection

Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include high density nucleic acid amplification and detection and immuno-PCR.

High density self-contained biological analysis

Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include high density nucleic acid amplification and detection and immune-PCR.

Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyzes include nucleic acid amplification and detection and immuno-PCR.

Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analysis include nucleic acid amplification and detection and immuno-PCR.

The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.

Annealing curve analysis in PCR

The present invention is directed to a nucleic acid quantification kit and method for determining the initial concentration or mass fraction of a target nucleic acid present in a sample. Illustrative embodiments include real-time competitive quantitative polymerase chain reaction (PCR) to determine the copy number or mass fraction of a target nucleic acid sequence in a sample and use of a thermodynamically based signal processing algorithm, with or without PCR, to provide mass fraction information.

Real-time gene quantification with internal standards

The present invention is directed to kits for monitoring a nucleic acid during amplification. More particularly, the present invention relates to kits comprising a first oligonucleotide labeled with a first fluorophore, a second oligonucleotide labeled with a second fluorophore, and components for amplifying the locus, wherein the first and second fluorophores comprise a fluorescence resonance energy transfer pair. Hybridization of at least the first oligonucleotide to the amplified locus places the first and second fluorophores in a resonance energy transfer relationship.

Monitoring amplification with fret probes

The invention provides a method for quantitative multiplex PCR of two targets whereby one target is present in at least 100-fold molar excess over that of the other target. The method further comprises the addition of a thermostable DNA polymerase with a final concentration of at least 0.5 units/&#x3bc;l.

Quantitative multiplex PCR with high dynamic range

Growing community of inventors

Salt Lake City, UT, United States of America

Randy P Rasmussen