Becton Dickinson and Company Limited

Randy J Somerville

Thomas D Haubert

James A Prescott

Michael L Marshall

Gregory S Kramer

Melody M H Kuroda

M Scott Ulrich

Dwight Livingston

Michael Justin Lizzi

Scott Nicholas Danhof

Ammon David Lentz

Mark A Talmer

Robert L Campbell

Jack H Kessler

Artis R Lockhart

Donald E Gorelick

Ernie Ritchey

Jeffrey S Ojala

Spencer Lovette

Scott N Danhof

Miroslaw Bartkowiak

Jack Kessler

Kristin Weidemaier

Adam C Curry

Andrea Liebmann-Vinson

Eric Fallows

Kevin G Dolan

David S Sebba

Wendy Dale Woodley

Erin Gooch Carruthers

Eric A Fallows

Melody MH Kuroda

Melody Mh Kuroda

David S Sebra

Vessel assembly

Provided herein are methods, systems, and devices for detecting and/or identifying one or more specific microorganisms in a culture sample. Indicator particles, such as surface enhanced Raman spectroscopy (SERS)-active nanoparticles, each having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest, can form a complex with specific microorganisms in the culture sample. Further, agitating magnetic capture particles also having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest can be used to capture the microorganism-indicator particle complex and concentrate the complex in a localized area of an assay vessel for subsequent detection and identification. The complex can be dispersed, pelleted, and redispersed so that the culture sample can be retested a number of times during incubation so as to allow for real-time monitoring of the culture sample.

Methods, systems, and devices for detecting and identifying microorganisms in microbiological culture samples

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Columbus, OH, United States of America

Randy J Somerville