Genia Technologies, Inc.

Roche Sequencing Solutions, Inc.

Randy Davis

Roger J A Chen

Roger Chen

Jeffrey R Davis

Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.

Systems and methods for forming a nanopore in a lipid bilayer

A sub-surface pole and pole foundation remover having a portable frame, a cylinder mounted on the frame and configured to receive pressurized fluid, a lifting piston slidably mounted in the cylinder, a grapnel connected to the lifting piston and having a plurality of lower arms that extend from a bottom circular support and that are configured to grip a sub-surface pole located below ground, and a control lever configured to direct the pressurized fluid to the cylinder that is actuated to cause the lifting piston to extend and push the grapnel upward relative to the portable frame to remove the pole from the ground. The grapnel may have a top circular support and a middle circular support connected to the top circular support by a plurality of flexible cables. A cinching cable may be used to grip the pole, the grapnel may have gripping teeth and fluid may be hydraulic.

Sub-surface post remover

A method of forming a nanopore in a lipid bilayer is disclosed. A nanopore forming solution is deposited over a lipid bilayer. The nanopore forming solution has a concentration level and a corresponding activity level of pore molecules such that nanopores are substantially not formed un-stimulated in the lipid bilayer. Formation of a nanopore in the lipid bilayer is initiated by applying an agitation stimulus level to the lipid bilayer. In some embodiments, the concentration level and the corresponding activity level of pore molecules are at levels such that less than 30 percent of a plurality of available lipid bilayers have nanopores formed un-stimulated therein.

Systems for forming a nanopore in a lipid bilayer

Methods for forming a nanopore in a lipid bilayer

Techniques for assembling a lipid bilayer on a substantially planar solid surface are described herein. In one example, a lipid material such as a lipid suspension is deposited on a substantially planar solid surface, a bubble filled with fast diffusing gas molecules is formed on the solid surface, and the gas molecules are allowed to diffuse out of the bubble to form a lipid bilayer on the solid surface.

Systems and methods for assembling a lipid bilayer on a substantially planar solid surface

Techniques for characterizing a molecule are described herein. In one example, a portion of the molecule is trapped in a nanopore, a variable voltage is applied across the nanopore until the trapped portion of molecule is moved within the nanopore, and the molecule is characterized based on the electrical stimulus required to affect movement of at least a portion of the trapped portion of the molecule within the nanopore.

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Pleasanton, CA, United States of America

Randy Davis