Wisconsin Alumni Research Foundation

Randolph Scott Ashton

Ethan S Lippmann

Neha Sehgal

Carlos Ruben Marti-Figueroa

Gavin T Knight

Described herein are chemically defined, adherent culture protocols for generating functional motor neurons characteristic of diverse hindbrain and spinal cord regions, with high efficiency.

Methods for efficient derivation of human motor neurons from diverse spinal regions

Described herein are methods, compositions, and kits for directed differentiation of human pluripotent stem cells, neuromesodermal progenitors, and neural stem cells into biomimetic neural tissues comprising one or more rosette structures. Preferably, the methods provided herein direct differentiation of human pluripotent stem cells, neuromesodermal progenitors, and neural stem cells into biomimetic neural tissues comprising a singular neural rosette structure that is comparable to at least a portion of the developing human neural tube. Also described are engineered neural tissue preparations comprising biomimetic neural tissues comprising a singular rosette structure having regional neural progenitor phenotypes.

Methods and culture substrates for controlled induction of biomimetic neural tissues comprising singular rosette structures

Described herein are methods, compositions, and kits for forming engineered in vitro biomimetic, three-dimensional, tubular organoid structures by directed differentiation of human pluripotent stem cells within tubular channels formed in a hydrogel.

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Madison, WI, United States of America

Randolph Scott Ashton