Illumina Corporation

Illumina Cambridge Limited

Randall Smith

Wenyi Feng

Bernard Hirschbein

Tarun Kumar Khurana

Xiaohai Liu

Xiaolin Wu

Joseph Francis Pinto

Poorya Sabounchi

Tracy Helen Fung

Helmy A Eltoukhy

Jonathan Mark Boutell

Robert C Kain

Geoffrey Paul Smith

Min-Jui Richard Shen

Kay Klausing

Thomas J Joseph

Carolyn Tregidgo

Wayne N George

Xiaodong Zhao

Ryan Christopher Smith

Steven Modiano

Dajun Yuan

John M Beierle

Peyton Shieh

Maxwell Zimmerley

M Shane Bowen

Andrew Antony Brown

Hai Quang Tran

Lewis J Kraft

Brian Douglas Mather

Liangliang Qiang

Michael S Graige

Steven H Modiano

Wei Wei

Arthur Pitera

Naiqian Zhan

Alexander Fuhrmann

Jie Mao

Silke Musa

Sara Bakhshi

Gerald Kreindl

Elliot John Lawrence

Thomas Joseph

Liangliang Quang

Arthur J Pitera

In one example, a method of preparing a fluidic channel includes covalently coupling a first region of a substrate to a first region of a cover using first moieties covalently coupled to the first region of the substrate and second moieties covalently coupled to the first region of the cover. The covalent coupling between the first region of the substrate and the first region of the cover suspends a second region of the cover over a second region of the substrate to form a fluidic channel.

Fluidic devices including fluidic channels, and methods of making the same

A method for patterning flow cell substrates using photo-initiated chemical reactions that includes fabricating a planar waveguide flow cell by forming a layer of light coupling gratings on a glass substrate layer; depositing a core layer on the layer of light coupling gratings; depositing a cladding layer on the core layer; and forming nanowells in the cladding layer; silanizing the cladding layer; coating the silanized cladding layer and nanowells with a first group of reactants; introducing a second group of reactants into the nanowells, wherein the second group of reactants includes a target reactant and a light-sensitive photoinitiator system; coupling a light source to the light coupling gratings and directing light internally within the planar waveguide flow cell for photo-initiating a chemical reaction between the first and second groups of reactants, wherein the photo-initiated chemical reaction covalently binds the target reactant to only the bottom portion of each nanowell.

System and method for patterning flow cell substrates

There is set forth herein a device comprising structure defining a detector surface configured for supporting biological or chemical substances, and a sensor array comprising light sensors and circuitry to transmit data signals using photons detected by the light sensors. The device can include one or more features for reducing fluorescence range noise in a detection band of the sensor array.

Detector with reduced fluorescence range noise

Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by NaSO. Nucleotides and oligonucleotides comprising a 3' phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

Compositions and methods for chemical cleavage and deprotection of surface-bound oligonucleotides

An interposer for a flow cell comprises a base layer having a first surface and a second surface opposite the first surface. The base layer comprises black polyethylene terephthalate (PET). A first adhesive layer is disposed on the first surface of the base layer. The first adhesive layer comprises methyl acrylic adhesive. A second adhesive layer is disposed on the second surface of the base layer. The second adhesive layer comprises methyl acrylic adhesive. A plurality of microfluidic channels extends through each of the base layer, the first adhesive layer, and the second adhesive layer.

Interposer with first and second adhesive layers

In one example, an unsaturated cyclic dione is coupled to the substrate, and is reacted with an indole or indazole including a first functional group to form a first adduct coupling the first functional group to the substrate. In another example, an unsaturated cyclic dione is coupled to a substrate and reacted with a diene including a functional group to form an adduct coupling the functional group to the substrate. In another example, an indole or indazole is coupled to a substrate, and is reacted with an unsaturated cyclic dione including an oligonucleotide to form an adduct coupling the oligonucleotide to the substrate. In another example, a diene is coupled to a substrate, and is reacted with an unsaturated cyclic dione including an oligonucleotide to form an adduct coupling the oligonucleotide to the substrate.

Compositions including functional groups coupled to substrates, and methods of making the same

An array includes a support including a plurality of discrete wells, a gel material positioned in each of the plurality of discrete wells, and a quality control tracer grafted to the gel material in each of the plurality of discrete wells. The quality control tracer comprises (a) a cleavable nucleotide sequence comprising a cleavage site and (b) a detectable label; and in some aspects, is a cleavable nucleotide sequence with a detectable label and a non-reactive nucleotide sequence or a primer nucleotide sequence.

Arrays with quality control tracers

The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

Methods and compositions for nucleic acid sequencing

A compound has Formula I: A, B, C, D, W, X, Y, and Z are independently selected from hydrogen, optionally substituted C-Calkyl, optionally substituted C-Calkoxy, aryl, aldehyde, protected aldehyde, CH, N, O, S, null, and bond; Q is selected from aldehyde, protected aldehyde, and null, at least one of A, B, C, D, W, X, Y, Z, or Q is an aldehyde or protected aldehyde; the bonds between each of A-B, B-C, C-D, W-X, X-Y, and Y-Z are selected from single bond, double bond, triple bond, and no bond; L is a linker selected from a C-Calkyl, aralkyl, and aryl, any of which is optionally substituted; one or more methylene unit (CH) of the C-Calkyl is optionally replaced by any combination of oxygen, carbonyl(C&#x2550;O), and NH; and Rand Rare independently selected from &#x2014;NRR, halogen, C-Calkoxy, aralkoxy, alkenyloxy, alkynyloxy, and OCHCHCN; Rand Rare independently a C-C, straight chain or branched alkyl group.

Formylpyrrole-based heterocycles for nucleic acid attachment to supports

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San Diego, CA, United States of America

Randall Smith