I-stat Corporation

Other

Randall M Mier

Graham Davis

Imants R Lauks

Sylvia Piznik

Henry J Wieck

Stephen N Cozzette

Paul Van Der Werf

Nicolaas Smit

Susan J Steiner

Jeanne A Itak

An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments. The present invention also discloses a novel method for the electrochemical detection of particular analyte species of biological and physiological significance using a substrate/label signal generating pair which produces a change in the concentration of electroactive species selected from the group consisting of dioxygen and hydrogen peroxide.

System and method of microdispensing and arrays of biolayers provided by same

An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments. The present invention also discloses a novel method for the electrochemical detection of particular analyte species of biological and physiological significance using an substrate/label signal generating pair which produces a change in the concentration of electroactive species selected from the group consisting of dioxygen and hydrogen peroxide.

Methods for microdispensing patterened layers

Process for the manufacture of wholly microfabricated biosensors

A method of forming a permselective layer on preselected areas of a substantially planar sensing device is disclosed. The claimed method includes establishing and confining a liquid film, derived from a silane compound mixed in a suitable solvent, within a predetermined area of the sensing device. The process relates to photolithographic imaging and developing methods coupled to a film-curing step that provides a patterned permselective layer having the desired semipermeable characteristics.

Method of forming a permselective layer

Wholly microfabricated biosensors and process for the manufacture and

A plurality of uniform microfabricated sensing devices are produced by establishing a plurality of base sensors on a substrate wafer, forming over at least a portion of each base sensor a permselective layer, superimposing a photoformable proteinaceous photoresist layer over a substantial portion of the permselective layer, and forming a topmost layer of an immobilized ligand receptor. The ligand receptor and corresponding ligand may be immunoreactive species.

Method of manufacturing a plurality of uniform microfabricated sensing

A rollable device, designed to be pushed by a blind person, has a plurality of sensors so spaced and so located upon a wheeled mount as to detect the presence of an object, step, or depression in the path of the user, and in so doing actuate electrical means designed to instantaneously inform the user of the presence of the potentially dangerous condition. The electrically actuated means includes vibratory devices in the handle of the device, so located as to acquaint a blind person with the locaton and nature of the dangerous condition in his or her path. Means is also incorporated in the device, in the form of a buzzer or other electrically actuated audible device, signifying the presence of objects such as automobile bumpers which are elevated above the ground surface and would therefore otherwise go undetected. Incorporated in the device, also, is a means actuable by the user for sounding a horn or the like for the purpose of seeking help or for warning others of his approach. The invention incorporates side and front bumpers, an antenna-like member, and a wheel suspension that drops bodily, as sensors having the capability of detecting a wide variety of dangerous conditions that may be encountered by a blind person.

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Morrisville, PA, United States of America

Randall M Mier