USA as Represented by Secretary of the Navy

US Government as Represented by the Secretary of the Army

Randal J Schoepp

Thomas J Palys

Kara E Schmid

Neal G Satterly

Disclosed herein methods, compositions, and kits for obtaining a normalized measured amount of a target in a sample by measuring the amount of the target in the sample by contacting the sample with a detection reagent, measuring the amount of a ligand known to be absent in the sample being tested by contacting the sample with a control reagent, and obtaining the normalized measured amount of the target by calculating the ratio of the measured amount of the target to the measured amount of the ligand.

Methods for normalizing background effects in multiplex immunoassays

In this application is described the production of recombinant, chimeric, humanized antibodies specific for both BoNt/A and BoNT/B. The humanized antibodies were converted from a mouse anti-BoNT/A/B Fab fragment into a whole human IgG1 antibody. The antibodies are useful in assays for detecting human exposure to BoNT/A and BoNT/B.

Growing community of inventors

Frederick, MD, United States of America

Randal J Schoepp