Akzo Nobel N.v.

Other

Biom&eacute;rieux, Inc.

University of Massachusetts

Advanced Bioscience Laboratories, Inc.

Ranajit Pal

Mangalasseril G Sarngadharan

Anthony L Devico

Anthony DeVico

Joseph W Romano

Robert C Gallo

Shan Lu

Shixia Wang

Alfredo Garzino-Demo

Phillip D Markham

Roxanne Shurtliff

Stephen Whitney

Vaniambadi Kalyanaraman

Balachandran Nair

Phillip Markham

Tim Keen

Stephen Charles Whitney

Balachandran C Nair

V S Kalyanaraman

Polyvalent, primary isolate nucleic acid compositions for inducing an immune response against HIV is disclosed. The composition and methods described herein are for the use of a DNA composition that encodes one or more different HIV envelope glycoproteins. The DNA composition can encode an HIV Gag protein. The DNAs encoding one or more HIV proteins are a combination of different nucleic acids, such as DNA plasmids, generated from primary isolate DNA of different HIV major group genetic clades and/or different proteins. HIV protein compositions for inducing an immune response against HIV are disclosed. Methods fro using the protein compositions as boosts following administration of the DNA compositions are provided.

Polyvalent, primary HIV-1 glycoprotein DNA vaccines and vaccination methods

The present invention relates to therapeutic compositions of macrophage derived chemokine (MDC) and methods for treating and preventing infection by a lentivirus, particularly an immunodeficiency virus, particularly HIV, using MDC proteins, nucleic acids and/or derivatives or analogues thereof. The present invention further relates to methods for detection and prognosis of lenivirus infection, particularly HIV infection using MDC as a prognostic indicator. The present invention further provides MDC proteins, nucleic acids encoding such proteins, that have amino-terminal sequences that differ from other MDC isolates. Recombinant host cells and methods of production are also provided.

Macrophage derived chemokine (MDC) as an anti-viral agent for the treatment and prevention of lentivirus infection

The present invention is directed to the discovery of antibodies that will react to proteins or nucleic acids bound to particular crosslinkers, but not to the free crosslinkers or free proteins or nucleic acids. Monoclonal antibodies with such binding specificity have widespread applications in receptor-ligand binding, immunodiagnostic and nucleic acid diagnostics.

Antibodies to crosslinkers and methods for using the same

A vaccine and a method of raising neutralizing antibodies against HIV infection. The vaccine comprises a complex of gp120 covalently bonded to CD4 or to succinyl concanavalin A. Also disclosed are immunological tests using the complex or antibody thereto for detection of HIV infection.

HIV immunogenic complexes comprising GP120 covalently bound to CD4 or concanavalin A

The present invention is directed to a method of raising neutralizing antibodies against HIV using a complex of gp120 covalently bonded to CD4 or a complex of gp120 covalently bonded to succinyl concanavalin A.

Method of raising neutralizing antibodies against HIV

An isothermal transcription based amplification assay for MDC RNA uses primer combinations for sequences within the MDC gene. A quantitative control uses a mutant RNA for comparison.

Isothermal transcription based amplification assay for the detection and

Neutralizing antibodies against HIV infection are provided. The antibodies are reactive with cryptic epitopes on gp120 and/or CD4 induced by the formation of immunogenic complexes comprising gp120 covalently bonded to CD4 or to succinyl concanvalin A.

Neutralizing antibodies against HIV infection

HIV immunogenic complexes

A method for determining virus replication in human cells by human retrovirus using RNA amplification comprising detecting the hybridization of an RNA probe which specifically hybridizes with spliced RNA and not with genomic RNA. This method permits early detection of RNA replication resulting from primary infection without detecting non-replicating virus.

Detection of human retrovirus infection

A vaccine and a method of raising neutralizing antibodies against HIV infection. The vaccine comprises a complex of gp120 bonded covalently to CD4 or to succinyl concanavalin A.

Growing community of inventors

Gaithersburg, MD, United States of America

Ranajit Pal