Glykos Finland Oy

Ramon Wahl

Jari Natunen

Markku Saloheimo

Anne Huuskonen

Jukka Hiltunen

Christian Ostermeier

Benjamin Patrick Sommer

Anne Kanerva

Juhani Saarinen

Ann Westerholm-Parvinen

Christopher Landowski

Bernhard Helk

Annamari Heiskanen

Eero Mustalahti

Anne Leppänen

Heidi Salminen

Georg Schmidt

The present disclosure relates to compositions and methods useful for the production of heterologous proteins with reduced O-mannosylation in filamentous fungal cells, such ascells. More specifically, the invention provides a PMT-deficient filamentous fungal cell comprising a) at least a first mutation that reduces an endogenous protease activity compared to a parental filamentous fungal cell which does not have said first mutation, and, b) at least a second mutation in a PMT gene that reduces endogenous O-mannosyltransferase activity compared to a parental filamentous fungal cell which does not have said second mutation, wherein said filamentous fungal cell is selected from the group consisting oforcell.

O-mannosyltransferase deficient filamentous fungal cells and methods of use thereof

The present disclosure relates to compositions and methods useful for the production of recombinant glycoproteins in filamentous fungal cells, such ascells, wherein at least 90% (mol %), preferably at least 95% of the total neutral N-glycans of said produced recombinant glycoprotein are mammalian-like N-glycans. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutations that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s); ii. a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase; iii. a recombinant polynucleotide for increasing &#x3b1;1, 2 mannosidase activity;and, iv. a recombinant polynucleotide encoding said heterologous glycoprotein.

Production of glycoproteins with mammalian-like N-glycans in filamentous fungi

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Basel, Switzerland

Ramon Wahl