Beckman Coulter, Inc.

Ramon Simon-Lopez

Robert T Magari

Cheng Qian

Ziling Huo

New diagnostic parameters or indexes for detection of absolute iron deficiency, latent iron deficiency, functional iron deficiency, or latent functional iron deficiency have been disclosed. The parameters include a RBC size factor, RSfdefined by the formula of (MCV&#xd7;MRV), or RSfdefined by the formula of (MCV&#xd7;MRV)/100, a volume-hemoglobin factor (VHf) defined by the formula of (MCV&#xd7;Hgb)/100, and a volume-hemoglobin/distribution factor (VHDWf) defined by the formula of (MCV&#xd7;Hgb)/(RDW&#xd7;10). Further disclosed are the methods of using these parameters for detection of absolute iron deficiency, latent iron deficiency, functional iron deficiency, or latent functional iron deficiency. Also disclosed is a method of using RSf for detection of hemochromatosis.

Methods of determination of iron deficiency and hemochromatosis

Methods of detection of iron deficiency and hemochromatosis

Methods for determining the responsiveness of a patient to recombinant human erythropoietin therapy are disclosed. In one approach, the method includes obtaining a volume-Hgb factor (VHf) defined as a product function of MCV and Hgb from a patient's blood sample; comparing VHf to a predetermined criterion; and reporting indication of responder if VHf meets the predetermined criterion. The method further includes using a RBC size-hemoglobin factor (RSHf) defined as a product function of MCV, MRV and Hgb, or using a function of VHf and RDW for determining the responsiveness. In another approach, the method includes using a RBC size factor (RSf) defined as a product function of MCV and MRV, in combination with transferrin saturation (TSAT) for determining the responsiveness to the erythropoietin therapy.

Methods of determination of responsiveness to erythropoietin treatment

A method of producing low hemoglobin concentration cell percentage (LHCC %) and the method of use for detection of iron deficiency have been disclosed. LHCC % is a sigmoid function of mean cell hemoglobin concentration (MCHC), which correlates linearly with hypochromic red cell percentage.

Low hemoglobin concentration cell percentage and method of use in detection of iron deficiency

New diagnostic indexes and the method of use for detection of iron deficiency have been disclosed. These include a logistic function of mean cell hemoglobin (MCH) and mean cell volume (MCV), and a logistic function of mean cell hemoglobin concentration (MCHC) and MCH.

Methods of detection of iron deficiency

A method of detecting parasite infection, particularly malaria, includes mixing a blood sample with a lytic reagent system to lyse red blood cells, and to form a sample mixture; performing a differential analysis of white blood cells of the sample mixture on a blood analyzer, and obtaining a cell volume distribution of a white blood cell subpopulation from a cell volume measurement used in the differential analysis; obtaining a cell volume parameter from the cell volume distribution of the white blood cell subpopulation; evaluating the cell volume parameter against a predetermined criterion, and reporting an indication of the parasite infection if the cell volume parameter meets the predetermined criterion. The method also uses a discriminant obtained from cell volume parameters of two different white blood cell subpopulations against a predetermined criterion. The method further use a cell distribution parameter of a cell distribution obtained using a RF impedance measurement.

Growing community of inventors

St. Cergue, Switzerland

Ramon Simon-Lopez