Oxford Nanopore Technologies Limited

Ramiz Iqbal Nathani

Lakmal Nishantha Jayasinghe

Mark Bruce

Stephen Robert Young

Luke McNeill

Pratik Raj Singh

Neil Roger Wood

Andrew John Heron

James White

James Anthony Clarke

Christopher James Wright

Rebecca Victoria Bowen

David Jackson Stoddart

Domenico Caprotti

Richard Muscat

Paul Richard Moody

Jessica Mary May Johnson

Jessica Mary May Knott

Mark John Bruce

The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using the mutant forms of lysenin.

Mutant pore

Methods of characterizing an analyte using a detector such as a nanopore and an enzyme are provided. One aspect features methods for characterizing a double-stranded polynucleotide using a detector, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

Method of characterizing a polynucleotide

Methods of characterizing an analyte using a nanopore. One aspect features methods for characterizing a double-stranded polynucleotide using a nanopore, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

Methods and systems for characterizing analytes using nanopores

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Oxford, United Kingdom

Ramiz Iqbal Nathani