Illumina Corporation

Epicentre Technologies Corporation

Cellscript, LLC

Ramesh Vaidyanathan

Scott Kuersten

Haiying Li Grunenwald

Molly Min He

Jerome J Jendrisak

Amirali Kia

Erin Bomati

Christian Gloeckner

Trina Faye Osothprarop

Darin J Haskins

Daniel Schlingman

Anupama Khanna

Joshua Burgess

Gary A Dahl

Igor Yu Goryshin

Mark A Maffitt

Bradley Baas

Agnes Radek

Ken Doyle

Ronald Meis

Igor Goryshin

Presented herein are methods and compositions for analyzing rare nucleic acid species. Some methods presented herein use DNA reassociation kinetics following thermal denaturation to define populations of nucleic acid sequences, e.g., highly abundant (e.g., cDNA from rRNA), moderately abundant, and less abundant or rare sequences (e.g., cDNA from mRNA).

Methods of analyzing nucleic acids

Transposomes and oligonucleotide replacement methods to make DNA libraries that have distinct 5' and 3&#x2032; tags, and to make directional libraries that are enriched for a desired strand.

Oligonucleotide replacement for di-tagged and directional libraries

Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.

Modified transposases for improved insertion sequence bias and increased DNA input tolerance

The present invention provides novel compositions, kits and methods employing RNA 5' polyphosphatases, RNA 5&#x2032; monophosphatases, capping enzymes, decapping enzymes, nucleic acid pyrophosphatases and RNA ligases, as well as other enzymes, for selective 5&#x2032; ligation tagging of desired classes of RNA molecules that differ with respect to particular chemical moieties on their 5&#x2032; ends. The 5&#x2032;  tagged RNA molecules can be used for synthesis of tagged first-stand cDNA, double-stranded cDNA, and sense or antisense RNA for a variety of uses.

Selective 5' ligation tagging of RNA

The present innovation provides methods and kits that enable rapid and efficient dual end-tagging of RNA to prepare libraries for analysis by applications such as next-generation RNA sequencing, qPCR, microarray analysis, or cloning. The methods do not require time-consuming and inefficient gel-purification steps that are common to methods known in the art. In addition, the present invention provides methods and kits for rapid, high-throughput enzymatic preparation of 5'-activated, 3&#x2032;-blocked DNA oligonucleotides from standard, single-stranded DNA oligonucleotides.

Methods and kits for 3'-end-tagging of RNA

The present invention relates to the discovery of RNA 5' polyphosphatase enzymes not previously described in the art, methods for discovery of said enzymes, compositions of said enzymes, methods for making said enzymes, and various methods and kits for using said enzymes for biomedical research, for human and non-human diagnostics, for production of therapeutic products, and for other applications. In particular, some embodiments provide compositions, kits and methods for employing RNA polyphosphatases for isolation, purification, production, and assay of capped RNA using a biological sample or a sample from an in vitro capping reaction wherein the sample also contains RNA that is not capped. Other embodiments provide compositions, kits and methods wherein RNA polyphosphatases comprise signal-amplifying enzymes for analyte-specific assays.

RNA polyphosphatase compositions, kits, and uses thereof

The present invention provides novel compositions, kits and methods employing RNA 5' polyphosphatases, RNA 5&#x2032; monophosphatases, capping enzymes, decapping enzymes, nucleic acid pyrophosphatases and RNA ligases, as well as other enzymes, for selective 5&#x2032; ligation tagging of desired classes of RNA molecules that differ with respect to particular chemical moieties on their 5&#x2032; ends. The 5&#x2032; tagged RNA molecules can be used for synthesis of tagged first-stand cDNA, double-stranded cDNA, and sense or antisense RNA for a variety of uses.

Growing community of inventors

Madison, WI, United States of America

Ramesh Vaidyanathan